Syntaxin 1A Antibody (1B11-1A8) - Azide and BSA Free

Novus Biologicals | Catalog # H00006804-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1B11-1A8

Format

Azide and BSA Free
View all Syntaxin 1A Primary Antibodies »

Product Specifications

Immunogen

STX1A (AAH03011.1, 1 a.a. ~ 251 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKDRTQELRTAKDSDDDDDVAVTVDRDRFMDEFFEQVEEIRGFIDKIAENVEEVKRKHSAILASPNPDEKTKEELEELMSDIKKTANKVRSKLKSIEQSIEQEEGLNRSSADLRIRKTQHSTLSRKFVEVMSEYNATQSDYRERCKGRIQRQLEITGRTTTSEELEDMLESGNPAIFASGIIMDSSISKQALSEIETRHSEIIKLENSIRELHDMFMDMAMLVESQTMWRGPCLTPRRPSSTRARRAGRKS

Specificity

STX1A - syntaxin 1A (brain)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Syntaxin 1A Antibody (1B11-1A8) - Azide and BSA Free

Western Blot: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02]

Western Blot: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02]

Western Blot: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02] - STX1A monoclonal antibody (M02), clone 1B11-1A8 Analysis of STX1A expression in IMR-32.
Immunocytochemistry/ Immunofluorescence: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02]

Immunocytochemistry/ Immunofluorescence: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02]

Immunocytochemistry/Immunofluorescence: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02] - Analysis of monoclonal antibody to STX1A on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02]

Western Blot: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02]

Western Blot: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02] - STX1A monoclonal antibody (M02), clone 1B11-1A8. Analysis of STX1A expression in PC-12.
Western Blot: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02]

Western Blot: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02]

Western Blot: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02] - Analysis of STX1A expression in transfected 293T cell line by STX1A monoclonal antibody (M02), clone 1B11-1A8.Lane 1: STX1A transfected lysate(29 KDa).Lane 2: Non-transfected lysate.
ELISA: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02]

ELISA: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02]

ELISA: Syntaxin 1A Antibody (1B11-1A8) [H00006804-M02] - Detection limit for recombinant GST tagged STX1A is approximately 0.1ng/ml as a capture antibody.

Applications for Syntaxin 1A Antibody (1B11-1A8) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Syntaxin 1A

Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone. Syntaxins function in this vesicle fusion process. Syntaxins also serve as a substrate for botulinum neurotoxin type C, a metalloprotease that blocks exocytosis and has high affinity for a molecular complex that includes the alpha-latrotoxin receptor (MIM 600565) which produces explosive exocytosis (Zhang et al., 1995 [PubMed 7622072]).[supplied by OMIM]

Alternate Names

Brain Syntaxin, HPC-1, p35-1, STX1A, SYN1A

Entrez Gene IDs

6804 (Human)

Gene Symbol

STX1A

OMIM

186590 (Human)

Additional Syntaxin 1A Products

Product Documents for Syntaxin 1A Antibody (1B11-1A8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Syntaxin 1A Antibody (1B11-1A8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Syntaxin 1A Antibody (1B11-1A8) - Azide and BSA Free

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Protocols

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FAQs

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