Recombinant Bovine Thrombopoietin R/Tpo R Protein

Novus Biologicals | Catalog # NBP3-57454

Novus Biologicals
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Key Product Details

Applications

Western Blot, ELISA, Cell Culture
View all Thrombopoietin R/Tpo R Proteins and Enzymes »

Product Specifications

Description

A recombinant protein corresponding to 332 aa of Bovine Thrombopoietin R/Tpo R.

Source: Yeast

Amino Acid Sequence: SPAPPACDPR LLNKLLRDSH VLHSRLSQCP DVNPLSTPVL LPVVDFSLGE WKTQTEQTKA QDVLGTTTLL LEAVMTARGQ LGPTCLSSLL VQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSAIFLS FQQLLRGKVR FLLLVVGPSL CAKRAPPATA VPGSISPLLT LNKLPNRTSG LLETNSSVSA RTTGFGLPNR LQGFRAKIPG LLNQTSRSLG QIPGHLNGTQ GPLSGVHGLF PGPSPRALGA PDIPLGTSDM GSLPPYLQPG DSPSPAHPPP GQYTLFSPSP TSPIPTVHLQ PLLPDPSAIT PNSSSSLLVA AHSHFRNLSQ EE (332)

Stable for up to 12 months from date of receipt at -20C. Stable for 3 months when stored in working aliquots with a carrier protein at -20C. Avoid repeated freeze/thaw cycles.

Purity

>98%, by SDS-PAGE

Endotoxin Level

No affinity tags & naturally endotoxin-free

Predicted Molecular Mass

34.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Enzyme-linked Immunosorbent Spot (ELISPOT)
ELISA Standard (Matched Pair) (ELISA(Sta))

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP3-57454
Formulation Lyophilized in 1-5X PBS containing 10% Trehalose
Preservative 10% Trehalose
Reconstitution Reconstitute in sterile PBS with at least 0.1% carrier protein
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thrombopoietin R/Tpo R

In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq]

Long Name

Thrombopoietin Receptor

Alternate Names

c-Mpl, CD110, MPL, MPLV, TpoR

Gene Symbol

MPL

Additional Thrombopoietin R/Tpo R Products

Product Documents for Recombinant Bovine Thrombopoietin R/Tpo R Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Bovine Thrombopoietin R/Tpo R Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Protocols

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