Tight Junction Protein 1 Antibody

Novus Biologicals | Catalog # NBP1-46111

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Peptide ELISA, Immunofluorescence, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Tight Junction Protein 1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PKPTSQNQFSEHDKT corresponding to internal region according to NP_003248.3, NP_783297.2.

Epitope

internal region

Marker

Intercellular Junctions/Tight Junction Marker

Specificity

This antibody is expected to recognize both reported isoforms (NP_003248.3; NP_783297.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Tight Junction Protein 1 Antibody

Tight Junction Protein 1 Antibody

Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-46111] -

Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-46111] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cellular junction staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Tight Junction Protein 1 Antibody

Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-46111] -

Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-46111] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for Tight Junction Protein 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:132000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Tight Junction Protein 1

Zona Occludens or tight junctions are plasma membrane structures that seal together the perimeters of polarized epithelial cell monolayers and provide the cellular barrier necessary to maintain vectoral secretion, absorption and transport; often defining luminal and intracellular space. Four tight junction specific proteins have been identified (ZO1, ZO2, ZO3 and occludin). ZO1 was the first protein shown to be specifically associated with the tight junction and remains the standard marker for these structures.

Alternate Names

DKFZp686M05161, MGC133289, Tight junction protein 1, tight junction protein 1 (zona occludens 1), tight junction protein ZO-1, TJP1, ZO1, ZO-1, zona occludens 1, Zona occludens protein 1, zonula occludens 1 protein, Zonula occludens protein 1

Entrez Gene IDs

7082 (Human)

Gene Symbol

TJP1

UniProt

NP_003248.3 (Human)

Additional Tight Junction Protein 1 Products

Product Documents for Tight Junction Protein 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Tight Junction Protein 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Tight Junction Protein 1 Antibody

Customer Reviews for Tight Junction Protein 1 Antibody

There are currently no reviews for this product. Be the first to review Tight Junction Protein 1 Antibody and earn rewards!

Have you used Tight Junction Protein 1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies