TOR/mTOR [p Ser2481] Antibody (9Y5P6)
Novus Biologicals | Catalog # NBP3-16907
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9Y5P6 expressed in HEK293
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Product Specifications
Immunogen
A synthetic phosphorylated peptide around S2481 of human TOR/mTOR (P42345). IHSFI
Modification
p Ser 2481
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit TOR/mTOR [p Ser2481] Antibody (9Y5P6) (NBP3-16907) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for TOR/mTOR [p Ser2481] Antibody (9Y5P6)
Western Blot: TOR/mTOR [p Ser2481] Antibody (9Y5P6) [NBP3-16907] - Western blot analysis of extracts of various cell lines, using TOR/mTOR (NBP3-16907) at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by IGF-1 (50 ng/mL) at 37C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.
Applications for TOR/mTOR [p Ser2481] Antibody (9Y5P6)
Application
Recommended Usage
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TOR
Long Name
Mammalian Target of Rapamycin
Alternate Names
FRAP1, FRAP2, MTOR, RAFT1
Gene Symbol
MTOR
Additional TOR Products
Product Documents for TOR/mTOR [p Ser2481] Antibody (9Y5P6)
Product Specific Notices for TOR/mTOR [p Ser2481] Antibody (9Y5P6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars