VAP-A Antibody (4C12) - Azide and BSA Free
Novus Biologicals | Catalog # H00009218-M01
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4C12
Format
Azide and BSA Free
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Product Specifications
Immunogen
VAPA (AAH02992, 1 a.a. ~ 242 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.MAKHEQILVLDPPTDLKFKGPFTDVVTTNLKLRNPSDRKVCFKVKTTAPRRYCVRPNSGIIDPGSTVTVSVMLQPFDYDPNEKSKHKFMVQTIFAPPNTSDMEAVWKEAKPDELMDSKLRCVFEMPNENDKLNDMEPSKAVPLNASKQDGPMPKPHSVSLNDTETRKLMEECKRLQGEMMKLSEENRHLRDEGLRLRKVAHSDKPGSTSTASFRDNVTSPLPSLLVVIAAIFIGFFLGKFIL
Specificity
VAPA - VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa (4C12)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Novus Biologicals Mouse VAP-A Antibody (4C12) - Azide and BSA Free (H00009218-M01) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-VAP-A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for VAP-A Antibody (4C12) - Azide and BSA Free
Western Blot: VAP-A Antibody (4C12) [H00009218-M01]
Western Blot: VAP-A Antibody (4C12) [H00009218-M01] - VAPA monoclonal antibody (M01), clone 4C12. Analysis of VAPA expression in human placenta.Immunocytochemistry/ Immunofluorescence: VAP-A Antibody (4C12) [H00009218-M01]
Immunocytochemistry/Immunofluorescence: VAP-A Antibody (4C12) [H00009218-M01] - Analysis of monoclonal antibody to VAPA on HeLa cell. Antibody concentration 10 ug/ml.Western Blot: VAP-A Antibody (4C12) [H00009218-M01]
Western Blot: VAP-A Antibody (4C12) [H00009218-M01] - VAPA monoclonal antibody (M01), clone 4C12. Analysis of VAPA expression in K-562.Western Blot: VAP-A Antibody (4C12) [H00009218-M01]
Western Blot: VAP-A Antibody (4C12) [H00009218-M01] - Analysis of VAPA expression in transfected 293T cell line by VAPA monoclonal antibody (M01), clone 4C12.Lane 1: VAPA transfected lysate(27.3 KDa).Lane 2: Non-transfected lysate.Applications for VAP-A Antibody (4C12) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: VAP-A
Long Name
VAMP [Vesicle-associated Membrane Protein]-associated Protein A
Alternate Names
VAMP-A, VAP-33, VAPA
Entrez Gene IDs
9218 (Human)
Gene Symbol
VAPA
UniProt
Additional VAP-A Products
Product Documents for VAP-A Antibody (4C12) - Azide and BSA Free
Product Specific Notices for VAP-A Antibody (4C12) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for VAP-A Antibody (4C12) - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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