Viral Dengue Virus 1/3 NS1 Antibody

Catalog # Availability Size / Price Qty
MAB94402-100
MAB94402-SP
Viral Dengue Virus 1/3 NS1 Antibody in Data
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Viral Dengue Virus 1/3 NS1 Antibody Summary

Species Reactivity
Viral
Specificity
In sandwich immunoassays, Dengue Virus 1/3 NS1 is detected.
Source
Monoclonal Mouse IgG2B Clone # 969977
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant Dengue Virus 3 NS1 protein
Asp1-Ala352
Accession # YP_001531169
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Dengue Virus NS1 Monoclonal Antibody (Catalog # MAB94401).


This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Dengue Virus 1/3 NS1 ELISA Standard Curve. Recombinant Dengue Virus 1 NS1 or Recombinant Dengue Virus 3 NS1 protein was serially diluted 2-fold and captured by Mouse Anti-Dengue Virus NS1 Monoclonal Antibody (Catalog # MAB94401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Dengue Virus 1/3 NS1 Monoclonal Antibody (Catalog # MAB94402) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dengue Virus 1/3 NS1

Dengue virus (DENV), the cause of Dengue fever, is a single positive-stranded RNA virus of the family Flaviviridae, genus Flavivirus. 5 closely related but antigenically distinct serotypes of the virus have been found: DENV-1, DENV-2, DENV-3, DENV-4 and DENV-5.  DENV has a genome of about 11,000 bases, encoding a single large polyprotein that is subsequently cleaved into several structural and non-structural peptides. The polyprotein is divided into three structural proteins, C, prM, E, seven non-structural proteins, NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5, and short non-coding regions on both the 5' and 3' ends. DENV nonstructural protein-1 (NS1) is a secreted glycoprotein that is absent from viral particles but accumulates in the supernatant and on the plasma membrane of cells during infection.

Long Name
Dengue Virus 1/3 Polyprotein
Entrez Gene IDs
5075725 (Viral)
Alternate Names
Dengue Virus 1/3 NS1

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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