Viral Dengue Virus 1/3 NS1 Antibody Summary
Accession # YP_001531169
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Dengue Virus NS1 Monoclonal Antibody (Catalog # MAB94401).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Dengue Virus 1/3 NS1 ELISA Standard Curve. Recombinant Dengue Virus 1 NS1 or Recombinant Dengue Virus 3 NS1 protein was serially diluted 2-fold and captured by Mouse Anti-Dengue Virus NS1 Monoclonal Antibody (Catalog # MAB94401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Dengue Virus 1/3 NS1 Monoclonal Antibody (Catalog # MAB94402) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dengue Virus 1/3 NS1
Dengue virus (DENV), the cause of Dengue fever, is a single positive-stranded RNA virus of the family Flaviviridae, genus Flavivirus. 5 closely related but antigenically distinct serotypes of the virus have been found: DENV-1, DENV-2, DENV-3, DENV-4 and DENV-5. DENV has a genome of about 11,000 bases, encoding a single large polyprotein that is subsequently cleaved into several structural and non-structural peptides. The polyprotein is divided into three structural proteins, C, prM, E, seven non-structural proteins, NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5, and short non-coding regions on both the 5' and 3' ends. DENV nonstructural protein-1 (NS1) is a secreted glycoprotein that is absent from viral particles but accumulates in the supernatant and on the plasma membrane of cells during infection.
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