Viral HHV8‑ORF74 APC‑conjugated Antibody

R&D Systems | Catalog # FAB5264A

R&D Systems
Discontinued Product
FAB5264A has been discontinued. View all HHV8-ORF74 products.

Key Product Details

Species Reactivity

Viral

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 462510
View all HHV8-ORF74 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with viral HHV8-ORF74
Met1-Thr342
Accession # AAB51506

Specificity

Detects viral HHV8-ORF74 in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Viral HHV8‑ORF74 APC‑conjugated Antibody

Detection of HHV8‑ORF74 antibody in HEK293 Human Cell Line Transfected with Viral HHV8-ORF74 and eGFP antibody by Flow Cytometry.

Detection of HHV8‑ORF74 in HEK293 Human Cell Line Transfected with Viral HHV8-ORF74 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with viral HHV8-ORF74 and eGFP was stained with either (A) Mouse Anti-Viral HHV8-ORF74 APC-conjugated Monoclonal Antibody (Catalog # FAB5264A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Applications for Viral HHV8‑ORF74 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with viral HHV8-ORF74 and eGFP

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: HHV8-ORF74

The herpes family represents about 130-140 double-stranded DNA viruses that infect almost all phyla in the animal kingdom. HHV8 (Human Herpes Virus-8) is one of eight herpes viruses known to infect humans. These eight viruses are categorized into three classes: alpha (HHV1-3 that infect neurons), beta (HHV5-7) and gamma (HHV4 and 8 that lie latent in B cells). HHV8 contains about 100 genes/ORFs, including viral homologues for MIP (vMIP), Bcl-2, IL-6, NCAM and IL-8 R2. HHV8-ORF74 is a homolog of IL-8 R2, and when expressed shows a 44-46 kDa G-protein coupled receptor that belongs to the rhodopsin/ beta -adrenergic family of GCPRs. Unlike IL-8 R2, HHV8-ORF74 is constitutively active. Its activity is regulated by multiple chemokines that can either upregulate basal activity (IL-8; GRO-alpha ), downregulate basal activity (IP-10; vMIP-II; SFD-1) or leave activity untouched (NAP-2; ENA-78), acting instead as a blocking factor. HHV8-ORF74 activation on infected endothelial cells appears to drive them into a spindle-shape, characteristic of that seen in Kaposi's sarcoma, and induces the expression of multiple proangiogenic molecules such as IL-6, IL-8, ICAM-1, VCAM-1 and VEGF plus VEGF R2. These effects appear to be necessary, but not necessarily sufficient, for Kaposi's sarcoma development. The ORF74 GPCR shares 29% and 27% amino acid sequence identity with human CXCR2 and CXCR1, respectively.

Long Name

Human Herpesvirus 8 ORF74

Alternate Names

HHV8ORF74, KSHV-vGPCR

Entrez Gene IDs

4961460 (Viral)

Gene Symbol

HHV8GK18_GP84

UniProt

AAB51506

Additional HHV8-ORF74 Products

Product Documents for Viral HHV8‑ORF74 APC‑conjugated Antibody

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Product Specific Notices for Viral HHV8‑ORF74 APC‑conjugated Antibody

For research use only

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