VPS35 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-13517
![Western Blot: VPS35 Antibody [NBP2-13517]](https://resources.rndsystems.com/images/products/VPS35-Antibody-Western-Blot-NBP2-13517-img0013.jpg "Western Blot: VPS35 Antibody [NBP2-13517]")
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: SCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDD
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for VPS35 Antibody - BSA Free
![Western Blot: VPS35 Antibody [NBP2-13517]](https://resources.rndsystems.com/images/products/VPS35-Antibody-Western-Blot-NBP2-13517-img0007.jpg "Western Blot: VPS35 Antibody [NBP2-13517]")
Western Blot: VPS35 Antibody [NBP2-13517]
Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.![VPS35 Antibody - BSA Free Western Blot: VPS35 Antibody - BSA Free [NBP2-13517]](https://resources.rndsystems.com/images/products/nbp2-13517_rabbit-polyclonal-vps35-antibody-295202516204517.jpg "Western Blot: VPS35 Antibody - BSA Free [NBP2-13517]")
Western Blot: VPS35 Antibody - BSA Free [NBP2-13517]
Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.![VPS35 Antibody - BSA Free Western Blot: VPS35 Antibody - BSA Free [NBP2-13517]](https://resources.rndsystems.com/images/products/nbp2-13517_rabbit-polyclonal-vps35-antibody-30520251019107.jpg "Western Blot: VPS35 Antibody - BSA Free [NBP2-13517]")
![VPS35 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NBP2-13517]](https://resources.rndsystems.com/images/products/nbp2-13517_-immunocytochemistry-immunofluorescence-639174076895596328.jpg "Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NBP2-13517]")
Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NBP2-13517]
Staining of human cell line U-2 OS shows localization to endosomes & lysosomes.Applications for VPS35 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04 - 0.4 ug/ml
Application Notes
ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: VPS35
Long Name
VPS35 retromer complex component
Alternate Names
MEM3, PARK17
Gene Symbol
VPS35
Additional VPS35 Products
Product Documents for VPS35 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for VPS35 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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