WT1 Antibody (6F-H2) - Azide and BSA Free

Western Blot: WT1 Antibody (6F-H2)Azide and BSA Free [NBP2-81012]

Western Blot: WT1 Antibody (6F-H2) - Azide and BSA Free [NBP2-81012] - Whole cell protein from human K562, HEK293 and kidney tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-WT1 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.

Immunohistochemistry: WT1 Antibody (6F-H2) - Azide and BSA Free [NBP2-81012]

Immunohistochemistry: WT1 Antibody (6F-H2) - Azide and BSA Free [NBP2-81012] - Staining of human kidney glomerulus tissue. Heat mediated antigen retrieval was performed by heating in citrate buffer (pH 6) at 95C for 20 minutes. Image from verified customer review. Image from the standard format of this antibody.

Western Blot: WT1 Antibody (6F-H2)Azide and BSA Free [NBP2-81012]

Western Blot: WT1 Antibody (6F-H2) - Azide and BSA Free [NBP2-81012] - Hypomethylation of the Intron 1 CpG island results in WT1 expression. RNA was isolated from K562 and U937 cells growing under atmospheric conditions (Normox) and from U937 cells growing in 1% O2 (Hypox) and was analyzed by RT-PCR using primers that span exon 5 of the WT1 mRNA. Ribosomal RNA 36B4 is used as a positive control, and molecular weight markers (MW) are shown. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0119837), licensed under a CC-BY license. Image from the standard format of this antibody.