Human xCT/SLC7A11 Antibody

R&D Systems | Catalog # MAB11251

R&D Systems
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1057408
View all xCT/SLC7A11 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within amino acids 1-50 of the Human xCT/SLC7A11 protein

Met1-Leu501
Accession # Q9UPY5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human xCT/SLC7A11 Antibody

Detection of Human xCT/SLC7A11 by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, MDA‑MB‑231 human breast cancer cell line, U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human xCT/SLC7A11 Monoclonal Antibody (Catalog # MAB11251) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for xCT/SLC7A11 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of xCT/SLC7A11 in HEK293 Human Cell Line Transfected with Human xCT/SLC7A11 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human xCT/SLC7A11 and eGFP was stained with (A) Mouse Anti-Human xCT/SLC7A11 Monoclonal Antibody (Catalog # MAB11251) or (B) Mouse IgG1 control antibody staining (MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human xCT/SLC7A11 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/mL
Sample:  HEK293 human embryonic kidney cell line transfected with human xCT/SLC7A11 and eGFP

Western Blot

2 µg/mL
Sample: Lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, MDA-MB-231 human breast cancer cell line, U2OS human osteosarcoma cell line.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: xCT/SLC7A11

xCT, also called SLC7A11, is the light chain component of the cysteine/glutamate amino acid exchange transporter system Xc (1,2). System Xc is composed of two subunits, the light chain (xCT) and the heavy chain (CD98hc, SLC3A2) and functions by cellular uptake of cysteine in exchange for glutamate in a 1:1 ratio (1,2). The human xCT gene is located on chromosome 4q28.3 and is synthesized as a 12-pass transmembrane protein with both the N- and C-terminals located intracellularly (2, 3). xCT is a 501 amino acids (aa) protein with a theoretical molecular weight of 55.4 kDa (3, 4). xCT expression serves many functional purposes in cells including redox balance, ferroptosis, and chemotherapy or cancer drug resistance (1-3, 5-7). Import of cysteine by xCT plays a role in promoting oxidative stress response as cysteine is a precursor for glutathione synthesis (2, 3, 5-7). Glutathione is a cofactor for ROS-detoxifying enzymes, including glutathione peroxidase (GPX), which help defend from cellular ROS-induced damage (2, 3, 5-7). In addition to its antioxidant role, xCT also utilizes glutathione and GPX to inhibit ferroptosis, which is iron-dependent, non-apoptotic cell-death that occurs with overproduction of lipid hydroperoxides (1-3, 5-7). As cancer cells often experience high oxidative stress, it is understandable that xCT is overexpressed in a variety of cancer types, such as acute myeloid leukemia and breast cancer, and affects cancer growth, invasion, metastasis, and prognosis (1-3, 5-7). xCT expression has also been shown to play a role in glutathione-mediated drug resistance during cancer treatment (1,5,7). However, studies have shown that xCT knockdown results in increased tumor cell death, highlighting its suitability as a druggable target (1,5,7). Specifically, the xCT inhibitors Sulfasalazine, an approved anti-inflammatory drug, and Erastin, a small molecule inhibitor, are potential therapeutic modalities for treating a variety of cancers when used in combination with radiotherapy or immunotherapy (1-3, 5-7).

References

  1.  Liu, J. Xia, X. & Huang, P. (2020). xCT: A Critical Molecule That Links Cancer Metabolism to Redox Signaling. Molecular therapy : the journal of the American Society of Gene Therapy. https://doi.org/10.1016/j.ymthe.2020.08.021.
  2.  Koppula, P. Zhang, Y. Zhuang, L. & Gan, B. (2018). Amino acid transporter SLC7A11/xCT at the crossroads of regulating redox homeostasis and nutrient dependency of cancer. Cancer communications. https://doi.org/10.1186/s40880-018-0288-x.
  3.  Lin, W. Wang, C., Liu, G., Bi, C., Wang, X. Zhou, Q., & Jin, H. (2020). SLC7A11/xCT in cancer: biological functions and therapeutic implications. American journal of cancer research.
  4. xCT: Uniprot (Q9UPY5).
  5. Koppula, P. Zhuang, L. & Gan, B. (2020). Cystine transporter SLC7A11/xCT in cancer: ferroptosis, nutrient dependency, and cancer therapy. Protein & cell. https://doi.org/10.1007/s13238-020-00789-5.
  6. Liu, L. Liu, R. Liu, Y. Li, G., Chen, Q. Liu, X., & Ma, S. (2020). Cystine-glutamate antiporter xCT as a therapeutic target for cancer. Cell biochemistry and function. https://doi.org/10.1002/cbf.3581.
  7. Cui, Q. Wang, J. Q. Assaraf, Y. G. Ren, L. Gupta, P. Wei, L., Ashby, C. R. Jr. Yang, D. H. & Chen, Z. S. (2018). Modulating ROS to overcome multidrug resistance in cancer. Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy. https://doi.org/10.1016/j.drup.2018.11.001.

Long Name

Cationic 1/Solute Carrier Family 7 Member 11

Alternate Names

CCBR1, SLC7A11

Entrez Gene IDs

23657 (Human); 26570 (Mouse); 310392 (Rat)

Gene Symbol

SLC7A11

UniProt

Q9UPY5 (Human)

Additional xCT/SLC7A11 Products

Product Documents for Human xCT/SLC7A11 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human xCT/SLC7A11 Antibody

For research use only

Related Research Areas

Infectious Diseases

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