Zika Virus (H/PF/2013) Envelope Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-13205

Novus Biologicals
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Key Product Details

Species Reactivity

Virus

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Zika Virus (H/PF/2013) Envelope Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of Zika Virus (H/PF/2013) Envelope (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

Reactivity Notes

Zika virus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Zika Virus (H/PF/2013) Envelope Antibody - BSA Free

Western Blot: Zika Virus (H/PF/2013) Envelope Antibody [NBP3-13205]

Western Blot: Zika Virus (H/PF/2013) Envelope Antibody [NBP3-13205]

Western Blot: Zika Virus (H/PF/2013) Envelope Antibody [NBP3-13205] - Non-infected (-) and infected (+) C6/36 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika Virus (H/PF/2013) Envelope antibody (NBP3-13205) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Zika Virus (H/PF/2013) Envelope Antibody [NBP3-13205]

Immunocytochemistry/ Immunofluorescence: Zika Virus (H/PF/2013) Envelope Antibody [NBP3-13205]

Immunocytochemistry/Immunofluorescence: Zika Virus (H/PF/2013) Envelope Antibody [NBP3-13205] - Immunofluorescent analysis of non-infected and infected vero cells using Zika Virus (H/PF/2013) Envelope antibody (NBP3-13205). Green: Zika Virus (H/PF/2013) Envelope antibody (NBP3-13205) diluted at 1:500.
Western Blot: Zika Virus (H/PF/2013) Envelope Antibody [NBP3-13205]

Western Blot: Zika Virus (H/PF/2013) Envelope Antibody [NBP3-13205]

Western Blot: Zika Virus (H/PF/2013) Envelope Antibody [NBP3-13205] - Mock and infected Vero whole cell extracts were separated by gradient gel, and the membrane was blotted with Zika Virus (H/PF/2013) Envelope antibody (NBP3-13205) diluted at 1:2500.
Western Blot: Zika Virus (H/PF/2013) Envelope Antibody [NBP3-13205]

Western Blot: Zika Virus (H/PF/2013) Envelope Antibody [NBP3-13205]

Western Blot: Zika Virus (H/PF/2013) Envelope Antibody [NBP3-13205] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika Virus (H/PF/2013) Envelope antibody (NBP3-13205) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Sandwich ELISA: Zika Virus (H/PF/2013) Envelope Antibody [NBP3-13205] - Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using NBP3-13587 as capture antibody at concentration of 5 ug/mL and NBP3-13205 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.
Sandwich ELISA: Zika Virus (H/PF/2013) Envelope Antibody [NBP3-13205] - Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using NBP3-13588 as capture antibody at concentration of 5 ug/mL and NBP3-13205 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.

Applications for Zika Virus (H/PF/2013) Envelope Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000-1:10000

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Sandwich ELISA

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Zika Virus (H/PF/2013) Envelope

Long Name

Zika Virus Polyprotein

Alternate Names

ZIKV-E, ZIKV-Env, ZIKV_E

Additional Zika Virus (H/PF/2013) Envelope Products

Product Documents for Zika Virus (H/PF/2013) Envelope Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Zika Virus (H/PF/2013) Envelope Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Infectious Diseases

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Protocols

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FAQs

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