ZNF174 Antibody (2D7-E9) - Azide and BSA Free

Novus Biologicals | Catalog # H00007727-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2D7-E9

Format

Azide and BSA Free
View all ZNF174 Primary Antibodies »

Product Specifications

Immunogen

ZNF174 (AAH00876, 1 a.a. ~ 234 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAKMEITLSSNTEASSKQERHIIAKLEEKRGPPLQKNCPDPELCRQSFRRFCYQEVSGPQEALSQLRQLCRQWLQPELHTKEQILELLVMEQFLTILPPEIQARVRHRCPMSSKEIVTLVEDFHRASKKPKQWVAVCMQGQKVLLEKTGSQLGEQELPDFQPQTPRRDLRESSPAEPSQAGAYDRLSPHHWEKSPLLQEPTPKLAGTELLIEKTDPNMATDELPCKLWLSFIA

Localization

Nuclear

Specificity

ZNF174 - zinc finger protein 174

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ZNF174 Antibody (2D7-E9) - Azide and BSA Free

Western Blot: ZNF174 Antibody (2D7-E9) [H00007727-M01]

Western Blot: ZNF174 Antibody (2D7-E9) [H00007727-M01]

Western Blot: ZNF174 Antibody (3E4) [H00007727-M01] - ZNF174 monoclonal antibody (M01), clone 2D7-E9 Analysis of ZNF174 expression in SW-13.
Immunocytochemistry/ Immunofluorescence: ZNF174 Antibody (2D7-E9) [H00007727-M01]

Immunocytochemistry/ Immunofluorescence: ZNF174 Antibody (2D7-E9) [H00007727-M01]

Immunocytochemistry/Immunofluorescence: ZNF174 Antibody (3E4) [H00007727-M01] - Analysis of monoclonal antibody to ZNF174 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: ZNF174 Antibody (2D7-E9) [H00007727-M01]

Western Blot: ZNF174 Antibody (2D7-E9) [H00007727-M01]

Western Blot: ZNF174 Antibody (3E4) [H00007727-M01] - Analysis of ZNF174 over-expressed 293 cell line, cotransfected with ZNF174 Validated Chimera RNAi ( Cat # H00007727-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZNF174 monoclonal antibody (M01), clone 2D7-E9 (Cat # H00007727-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: ZNF174 Antibody (2D7-E9) [H00007727-M01]

Western Blot: ZNF174 Antibody (2D7-E9) [H00007727-M01]

Western Blot: ZNF174 Antibody (3E4) [H00007727-M01] - Analysis of ZNF174 expression in transfected 293T cell line by ZNF174 monoclonal antibody (M01), clone 2D7-E9.Lane 1: ZNF174 transfected lysate(26 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: ZNF174 Antibody (3E4) [H00007727-M01] - Detection limit for recombinant GST tagged ZNF174 is approximately 0.3ng/ml as a capture antibody.

Applications for ZNF174 Antibody (2D7-E9) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ZNF174

ZNF174 is a transcriptional repressor. Expressed in a variety of organs, but most strongly in adult testis and ovary followed by small intestine, colon, prostate, thymus, spleen, pancreas, skeletal muscle, heart, brain and kidney. Also expressed in umbilical vein endothelial cells, foreskin fibroblast and HEPG2 cells.

Alternate Names

AW-1, zinc finger protein 174, ZSCAN8Zinc finger and SCAN domain-containing protein 8

Entrez Gene IDs

7727 (Human)

Gene Symbol

ZNF174

OMIM

603900 (Human)

UniProt

AAH00876 (Human)

Additional ZNF174 Products

Product Documents for ZNF174 Antibody (2D7-E9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ZNF174 Antibody (2D7-E9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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