14-3-3 beta/alpha Antibody (5B5G10) - BSA Free

Novus Biologicals | Catalog # NBP2-61780

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 5B5G10

Format

BSA Free
View all 14-3-3 beta/alpha Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human 14-3-3 beta/alpha (AA: 1-246) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 14-3-3 beta/alpha Antibody (5B5G10) - BSA Free

Western Blot: 14-3-3 beta/alpha Antibody (5B5G10)BSA Free [NBP2-61780]

Western Blot: 14-3-3 beta/alpha Antibody (5B5G10)BSA Free [NBP2-61780]

Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mAb against HEK293 (1) and YWHAB (AA: 1-246)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) - BSA Free [NBP2-61780]

Immunocytochemistry/ Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) - BSA Free [NBP2-61780]

Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of HepG2 cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry: 14-3-3 beta/alpha Antibody (5B5G10) - BSA Free [NBP2-61780]

Immunohistochemistry: 14-3-3 beta/alpha Antibody (5B5G10) - BSA Free [NBP2-61780]

Immunohistochemistry: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of paraffin-embedded endometrial cancer tissues using YWHAB mouse mAb with DAB staining.
Western Blot: 14-3-3 beta/alpha Antibody (5B5G10)BSA Free [NBP2-61780]

Western Blot: 14-3-3 beta/alpha Antibody (5B5G10)BSA Free [NBP2-61780]

Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mAb against human YWHAB (AA: 1-246) recombinant protein. (Expected MW is 54 kDa)
Western Blot: 14-3-3 beta/alpha Antibody (5B5G10)BSA Free [NBP2-61780]

Western Blot: 14-3-3 beta/alpha Antibody (5B5G10)BSA Free [NBP2-61780]

Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mouse mAb against Hela (1), NIH/3T3 (2), C6 (3), A431 (4), K562 (5), PC-12 (6), and U937 (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) - BSA Free [NBP2-61780]

Immunocytochemistry/ Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) - BSA Free [NBP2-61780]

Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of GC-7901 cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunocytochemistry/ Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) - BSA Free [NBP2-61780]

Immunocytochemistry/ Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) - BSA Free [NBP2-61780]

Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of Hela cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry: 14-3-3 beta/alpha Antibody (5B5G10) - BSA Free [NBP2-61780]

Immunohistochemistry: 14-3-3 beta/alpha Antibody (5B5G10) - BSA Free [NBP2-61780]

Immunohistochemistry: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of paraffin-embedded rectum cancer tissues using YWHAB mouse mAb with DAB staining.
ELISA: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
ELISA: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of Hela cells using YWHAB mouse mAb (green) and negative control (red).

Applications for 14-3-3 beta/alpha Antibody (5B5G10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3 beta/alpha

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.;

Alternate Names

14-3-3 alpha, 14-3-3 beta, 14-3-3 protein beta/alpha, brain protein 14-3-3, beta isoform, GW128, HS1, KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, protein kinase C inhibitor protein-1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alphapolypeptide, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, betapolypeptide, YWHAA

Gene Symbol

YWHAB

Additional 14-3-3 beta/alpha Products

Product Documents for 14-3-3 beta/alpha Antibody (5B5G10) - BSA Free

Certificate of Analysis

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Product Specific Notices for 14-3-3 beta/alpha Antibody (5B5G10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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