4-Hydroxy-2-hexenal Antibody (5C11.1) - BSA Free
Novus Biologicals | Catalog # NBP3-18263
Key Product Details
Species Reactivity
Non-species specific
Applications
Western Blot, ELISA, Dot Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5C11.1
Format
BSA Free
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Product Specifications
Immunogen
Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH)
Specificity
Specific for 4-HHE. Slight cross-reactivity with 4-Hydroxynonenal. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, MDA, or Methylglyoxal modified proteins.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for 4-Hydroxy-2-hexenal Antibody (5C11.1) - BSA Free
Dot Blot: 4-Hydroxy-2-hexenal Antibody (5C11.1) [NBP3-18263] - Dot blot analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 5C11.1 (NBP3-18263). Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody (NBP3-18263) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.
Applications for 4-Hydroxy-2-hexenal Antibody (5C11.1) - BSA Free
Application
Recommended Usage
ELISA
1:1000
Western Blot
1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS pH 7.4, 50% glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: 4-Hydroxy-2-hexenal
Alternate Names
4-HHE;4-hydroxy Hexenal;4-Hydroxy-2-hexenal;4-Hydroxy-2-hexenal-modified;HHE;MG;MG-modified protein
Additional 4-Hydroxy-2-hexenal Products
Product Documents for 4-Hydroxy-2-hexenal Antibody (5C11.1) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for 4-Hydroxy-2-hexenal Antibody (5C11.1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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