5-hmC Antibody (AB3/63.3) - BSA Free
Novus Biologicals | Catalog # NBP2-50099
Key Product Details
Species Reactivity
All Species
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Dot Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # AB3/63.3
Format
BSA Free
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Product Specifications
Immunogen
5-hmC conjugated to BSA
Specificity
Expected to detect 5-hydroxymethylcytosine in all species.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Applications for 5-hmC Antibody (AB3/63.3) - BSA Free
Application
Recommended Usage
Dot Blot
1:100 - 1:2000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunoprecipitation
1:10 - 1:500
Application Notes
Positive control(s): Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: 5-hmC
Alternate Names
5'-hmC, 5-Hydroxymethylcytosine
Additional 5-hmC Products
Product Documents for 5-hmC Antibody (AB3/63.3) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for 5-hmC Antibody (AB3/63.3) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for 5-hmC Antibody (AB3/63.3) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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