5-HT2B Antibody - BSA Free

Novus Biologicals | Catalog # NLS1187

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Chimpanzee (100%), Orangutan (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all 5-HT2B Primary Antibodies »

Product Specifications

Immunogen

Synthetic 20 amino acid peptide from N-terminal extracellular domain of human 5-HT2B.

Epitope

N-Terminus extracellular domain

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (95%), Canine (85%), Bovine (80%), Rabbit (80%).

Localization

Cell Membrane

Specificity

Human 5-HT2B. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for 5-HT2B Antibody - BSA Free

Immunohistochemistry-Paraffin: 5-HT2B Antibody - BSA Free [NLS1187]

Immunohistochemistry-Paraffin: 5-HT2B Antibody - BSA Free [NLS1187]

Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1187] - Brain, Amygdala neurons
Immunohistochemistry-Paraffin: 5-HT2B Antibody - BSA Free [NLS1187]

Immunohistochemistry-Paraffin: 5-HT2B Antibody - BSA Free [NLS1187]

Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1187] - Analysis of anti-5HT2B Receptor antibody with human brain, neurons and glia.
Immunohistochemistry-Paraffin: 5-HT2B Antibody - BSA Free [NLS1187]

Immunohistochemistry-Paraffin: 5-HT2B Antibody - BSA Free [NLS1187]

Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1187] - Anti-HTR1B / 5-HT2B Receptor antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: 5-HT2B Antibody - BSA Free [NLS1187]

Immunohistochemistry-Paraffin: 5-HT2B Antibody - BSA Free [NLS1187]

Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1187] - Human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: 5-HT2B Antibody - BSA Free [NLS1187]

Immunohistochemistry-Paraffin: 5-HT2B Antibody - BSA Free [NLS1187]

Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1187] - Brain, Substantia Nigra

Applications for 5-HT2B Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

3 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: 5-HT2B

5-HT2B, a Serotonin Receptor, activates phospholipase C upon binding serotonin, which results in a rise in intracellular calcium. It is known that 5-HT2B regulates cardiovascular function during development and in adulthood; mice lacking functional 5-HT2B receptors died of heart defects during gestation or neonatally, and adult mutant mice displayed cardiopathy, including myocyte disarray and ventricular dilation. Abnormal vascular proliferation causes an increase in pulmonary blood pressure, which is associated with an increase in 5-HT2B receptor, resulting in the development of pulmonary hypertension. It has also been reported that the 5-HT2B is involved in cell-cycle regulation via interaction with tyrosine kinase pathways. 5-HT2B receptors may have a role in the initiation of migraine headaches, and selective antagonists may prove therapeutic in migraine treatment. 5-HT2B receptor is expressed in many tissues including human liver, kidney, lung, heart, pancreas, spleen, brain, spinal cord, gastrointestinal tract, placenta, and coronary and pulmonary arteries. It has also been reported in embryonic mouse heart and spinal cord. ESTs have been isolated from heart/melanocyte/uterus, kidney, prostate, skin, and thyroid libraries.

Long Name

5-Hydroxytryptamine Receptor 2B

Alternate Names

5HT2B, HTR2B

Entrez Gene IDs

3357 (Human)

Gene Symbol

HTR2B

Additional 5-HT2B Products

Product Documents for 5-HT2B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for 5-HT2B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for 5-HT2B Antibody - BSA Free

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Protocols

View specific protocols for 5-HT2B Antibody - BSA Free (NLS1187):

Immunohistochemistry Protocol for 5HT2B Receptor Antibody (NLS1187):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4-um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes at 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene approximately 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol approximately 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol approximately 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol approximately 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water approximately 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block approximately 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody approximately 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T approximately 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary approximately 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T approximately 1 minute at RT.
17. Apply an alkaline phosphatase steptavidin approximately 30 minutes at RT.
18. Rinse the slide in 1X TBS-T approximately 1 minute at RT.
19. Apply an alkaline phosphatase chromagen substrate approximately 30 minutes at RT.
20. Rinse the slide in distilled water approximately 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol approximately 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol approximately 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol approximately 1 minute each @ RT.
24. Wash the slides in 3 changes of xyleneapproximately 1 minute each @ RT.
25. Apply cover slip.

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