5-MethylCytosine Antibody (4111) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-42814
![Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814]](https://resources.rndsystems.com/images/products/5-MethylCytosine-Antibody-4111-Immunohistochemistry-Paraffin-NBP2-42814-img0008.jpg "Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814]")
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Key Product Details
Species Reactivity
Validated:
All Species
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Whole-Mount, Methylated DNA Immunoprecipitation, Dot Blot, Electron Microscopy
Cited:
Immunohistochemistry-Paraffin, Dot Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 4111
Format
Azide and BSA Free
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Product Specifications
Immunogen
The immunogen used to generate this antibody corresponds to 5-MethylCytosine
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for 5-MethylCytosine Antibody (4111) - Azide and BSA Free
Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814]
Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Paraffin-embedded rat brain. 5-Methylcytosine / 5-mC antibody diluted at 1:200.![Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814]](https://resources.rndsystems.com/images/products/5-MethylCytosine-Antibody-4111-Immunohistochemistry-Paraffin-NBP2-42814-img0002.jpg "Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814]")
Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814]
Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of human ovarian carcinoma. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:200.![Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814]](https://resources.rndsystems.com/images/products/5-MethylCytosine-Antibody-4111-Immunohistochemistry-Paraffin-NBP2-42814-img0003.jpg "Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814]")
Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814]
Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of mouse testis. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:200.![Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814]](https://resources.rndsystems.com/images/products/5-MethylCytosine-Antibody-4111-Immunohistochemistry-Paraffin-NBP2-42814-img0004.jpg "Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814]")
Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814]
Immunohistochemistry-Paraffin: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of mouse urinary bladder. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:200.![Immunohistochemistry Whole-Mount: 5-MethylCytosine Antibody (4111) [NBP2-42814]](https://resources.rndsystems.com/images/products/5-MethylCytosine-Antibody-4111-Immunohistochemistry-Whole-Mount-NBP2-42814-img0005.jpg "Immunohistochemistry Whole-Mount: 5-MethylCytosine Antibody (4111) [NBP2-42814]")
Immunohistochemistry Whole-Mount: 5-MethylCytosine Antibody (4111) [NBP2-42814]
Immunohistochemistry Whole-Mount: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of 24 hours-post-fertilization zebrafish embryo. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:50.![5-MethylCytosine Antibody (4111) [NBP2-42814]](https://resources.rndsystems.com/images/products/5-MethylCytosine-Antibody-4111-Methylated-DNA-Immunoprecipitation-NBP2-42814-img0006.jpg "5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of 24 hours-post-fertilization zebrafish embryo. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:50.")
5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of 24 hours-post-fertilization zebrafish embryo. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:50.
Methylated DNA Immunoprecipitation: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of 24 hours-post-fertilization zebrafish embryo. 5-Methylcytosine / 5-mC antibody [4111] dilution: 1:50.
Dot Blot: 5-MethylCytosine Antibody (4111) [NBP2-42814] - Analysis of anti-5-mC antibody with the synthetic DNA controls. DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine.
Applications for 5-MethylCytosine Antibody (4111) - Azide and BSA Free
Application
Recommended Usage
Dot Blot
1:100 - 1:2000
Electron Microscopy
Assay dependent
Immunohistochemistry
1:100-1:1000
Immunohistochemistry Whole-Mount
1:50-1:500
Immunohistochemistry-Frozen
Assay dependent
Immunohistochemistry-Paraffin
1:100-1:1000
Methylated DNA Immunoprecipitation
Assay dependent
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: 5-MethylCytosine
Alternate Names
5-mC, 5-Methyl Cytosine, Cytosine (5-Methyl)
Additional 5-MethylCytosine Products
Product Documents for 5-MethylCytosine Antibody (4111) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for 5-MethylCytosine Antibody (4111) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for 5-MethylCytosine Antibody (4111) - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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