5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free

Novus Biologicals | Catalog # NBP2-48480

Clone AD2 was used by HLDA to establish CD designation.
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Flow (Cell Surface)

Cited:

Immunohistochemistry-Frozen, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AD2

Format

BSA Free
View all 5'-Nucleotidase/CD73 Primary Antibodies »

Product Specifications

Immunogen

Human CD73 [UniProt# P21589]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free [NBP2-48480]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free [NBP2-48480]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - Analysis of 5'-Nucleotidase/CD73 antibody on Human lung tissue. PFA fixed paraffin-embedded sections. Sections were incubated with the primary antibody overnight in 4C (1:200 dilution). Image from verified customer review.
Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free [NBP2-48480]

Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free [NBP2-48480]

Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on CD3+ human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25186PCP.
Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free [NBP2-48480]

Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free [NBP2-48480]

Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on U87 MG cells with 5'-Nucleotidase/CD73 Antibody (AD2) antibody NBP2-48480 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.
Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free [NBP2-48480]

Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free [NBP2-48480]

Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647.
Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free [NBP2-48480]

Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free [NBP2-48480]

Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A cell surface stain was performed on U87MG cells with NBP2-48480APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.

Applications for 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:500

Immunohistochemistry-Frozen

reported in scientific literature (PMID 1989648)

Immunohistochemistry-Paraffin

reported by customer review

Reviewed Applications

Read 1 review rated 5 using NBP2-48480 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5'-Nucleotidase/CD73

This AD2 monoclonal CD73 antibody reacts with human CD73, a 5'-ectonucleotidase that converts 5'-adenosine monophosphate to adenosine. CD73 is expressed on the surface of endothelial cells, as well as B and T cells, including some CD4+Foxp3+ regulatory T cells. Adenosine production by these cells has been linked to the inhibition of CD4 T cell effector functions such as proliferation and cytokine secretion.

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE, ad2 anti cd73, ad2 cd73

Gene Symbol

NT5E

Additional 5'-Nucleotidase/CD73 Products

Product Documents for 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free

Customer Reviews for 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • 5'-Nucleotidase/CD73 Antibody (AD2)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Lung tissue
    Species: Human
    Verified Customer | Posted 01/19/2022
    Human lung staining
    PFA fixed paraffin-embedded sections. Sections were incubated with the primary antibody overnight in 4C (1:200 dilution).
    5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free NBP2-48480
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A:

    We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies