5T4 Antibody (1B6) - Azide and BSA Free

Novus Biologicals | Catalog # H00007162-M09

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1B6

Format

Azide and BSA Free
View all 5T4 Primary Antibodies »

Product Specifications

Immunogen

TPBG (NP_006661, 219 a.a. ~ 328 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SNHFLYLPRDVLAQLPSLRHLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEK

Specificity

TPBG - trophoblast glycoprotein, (1B6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for 5T4 Antibody (1B6) - Azide and BSA Free

Western Blot: 5T4 Antibody (1B6) [H00007162-M09]

Western Blot: 5T4 Antibody (1B6) [H00007162-M09]

Western Blot: 5T4 Antibody (1B6) [H00007162-M09] - Analysis of TPBG expression in HeLa (Cat # L013V1).
Western Blot: 5T4 Antibody (1B6) [H00007162-M09]

Western Blot: 5T4 Antibody (1B6) [H00007162-M09]

Western Blot: 5T4 Antibody (1B6) [H00007162-M09] - Analysis of TPBG expression in PC-12 (Cat # L012V1).
Sandwich ELISA: 5T4 Antibody (1B6) [H00007162-M09] - Detection limit for recombinant GST tagged TPBG is approximately 1ng/ml as a capture antibody.
Sandwich ELISA: 5T4 Antibody (1B6) [H00007162-M09] - Detection limit for recombinant GST tagged TPBG is 0.3 ng/ml as a capture antibody.

Applications for 5T4 Antibody (1B6) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: 5T4

The 5T4 gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is ass

Long Name

Oncofetal Antigen/Trophoblast Glycoprotein

Alternate Names

M6P1, TPBG

Entrez Gene IDs

7162 (Human)

Gene Symbol

TPBG

UniProt

NP_006661

Additional 5T4 Products

Product Documents for 5T4 Antibody (1B6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for 5T4 Antibody (1B6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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FAQs

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