8-Bromo-cAMP, sodium salt

Tocris Bioscience | Catalog # 1140

Cell-permeable cAMP analog
Tocris Bioscience

Key Product Details

Description

Cell-permeable cAMP analog
View all cAMP Compounds »

Product Description

8-Bromo-cAMP, sodium salt is a cell-permeable cyclic AMP (cAMP) analog. It activates PKA and has greater resistance to hydrolysis by phosphodiesterase (PDE) than cAMP. It increases differentiation and induces apoptosis in an esophageal cancer cell line. 8-Bromo-cAMP enhances the induction of pluripotency in human fibroblast cells in combination with VPA (valproic acid) (Cat. No. 2815). In combination with IBMX (Cat. No. 2845), it promotes the differentiation of human iPS cell-derived intestinal epithelial cells. It induces cell-based VEGF production for angiogenesis and osteoblastic differentiation in vitro.

Product Specifications for 8-Bromo-cAMP, sodium salt

Molecular Weight

430.09

Formula

C10H10BrN5NaO6P

Storage

Store at -20°C

Purity

≥99% (HPLC)

Chemical Name

8-Bromoadenosine-3',5'-cyclic monophosphate sodium salt

CAS Number

76939-46-3

PubChem ID

23702958

InChI Key

DMRMZQATXPQOTP-GWTDSMLYSA-M

SMILES

[Na+].[H][C@@]12COP([O-])(=O)O[C@@]1([H])[C@@H](O)[C@@H](O2)N1C(Br)=NC2=C1N=CN=C2N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 43.01 100
DMSO 43.01 100

Preparing Stock Solutions for 8-Bromo-cAMP, sodium salt

The following data is based on the product molecular weight 430.09.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.33 mL 11.63 mL 23.25 mL
5 mM 0.47 mL 2.33 mL 4.65 mL
10 mM 0.23 mL 1.16 mL 2.33 mL
50 mM 0.05 mL 0.23 mL 0.47 mL

Calculators

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Product Documents for 8-Bromo-cAMP, sodium salt

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for 8-Bromo-cAMP, sodium salt

For research use only

Citations for 8-Bromo-cAMP, sodium salt

Customer Reviews for 8-Bromo-cAMP, sodium salt (2)

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  • 8-bromo-cAMP phosphorylates cardiac CaMKII
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: Cardiomyocytes
    Verified Customer | Posted 05/15/2019
    HL-1 cardiomyocytes were treated with 100 nM fostriecin, 100 nM Okadaic acid or 50 nM 8-bromo-cAMP for 30 min followed by Western blot analysis for pCaMKII expression. 8-bromo-cAMP increased phosphorylation of CaMKII; indicating role of cAMP in CaMKII dephosphorylation.
    8-Bromo-cAMP, sodium salt 1140
  • Worked well
    Name: Emma Jones
    Assay Type: In Vitro
    Cell Line/Tissue: iPSC derived human astrocytes
    Verified Customer | Posted 01/04/2018
    Cells treated in vitro

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