AAK1 Antibody

Novus Biologicals | Catalog # NBP1-45214

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all AAK1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GSPRTSQQNVYNPSE corresponding to internal region according to NP_055726.3.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for AAK1 Antibody

Western Blot: AAK1 Antibody [NBP1-45214]

Western Blot: AAK1 Antibody [NBP1-45214]

Western Blot: AAK1 Antibody [NBP1-45214] - (1ug/ml) Analysis of Human Amygdala lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for AAK1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 100 kDa band observed in human brain (amygdala and cerebellum) lysates (calculated MW of 104 kDa band according to NP_055726.3).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AAK1

Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Long Name

AP2-associated Protein Kinase 1

Alternate Names

Adaptor-associated kinase 1, AP2 associated kinase 1, AP2-associated protein kinase 1, DKFZp686F03202, DKFZp686K16132, EC 2.7.11.1, FLJ23712, FLJ25931, FLJ31060, FLJ45252, KIAA1048FLJ42882, MGC138170, MGC164568, MGC164570

Entrez Gene IDs

22848 (Human); 269774 (Mouse); 500244 (Rat)

Gene Symbol

AAK1

UniProt

NP_055726.3 (Human)

Additional AAK1 Products

Product Documents for AAK1 Antibody

Certificate of Analysis

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Product Specific Notices for AAK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

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