ABAT Antibody (OTI6C9) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70060

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6C9

Format

Azide and BSA Free
View all ABAT Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 29-323 of human ABAT(NP_065737) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

53.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ABAT Antibody (OTI6C9) - Azide and BSA Free

Western Blot: ABAT Antibody (OTI6C9)Azide and BSA Free [NBP2-70060]

Western Blot: ABAT Antibody (OTI6C9)Azide and BSA Free [NBP2-70060]

Western Blot: ABAT Antibody (OTI6C9) - Azide and BSA Free [NBP2-70060] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABAT.
Immunohistochemistry: ABAT Antibody (OTI6C9) - Azide and BSA Free [NBP2-70060]

Immunohistochemistry: ABAT Antibody (OTI6C9) - Azide and BSA Free [NBP2-70060]

Immunohistochemistry: ABAT Antibody (OTI6C9) - Azide and BSA Free [NBP2-70060] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)
Immunohistochemistry: ABAT Antibody (OTI6C9) - Azide and BSA Free [NBP2-70060]

Immunohistochemistry: ABAT Antibody (OTI6C9) - Azide and BSA Free [NBP2-70060]

Immunohistochemistry: ABAT Antibody (OTI6C9) - Azide and BSA Free [NBP2-70060] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: ABAT Antibody (OTI6C9) - Azide and BSA Free [NBP2-70060]

Immunohistochemistry: ABAT Antibody (OTI6C9) - Azide and BSA Free [NBP2-70060]

Immunohistochemistry: ABAT Antibody (OTI6C9) - Azide and BSA Free [NBP2-70060] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for ABAT Antibody (OTI6C9) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ABAT

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq]

Long Name

4-aminobutyrate aminotransferase, mitochondrial

Alternate Names

EC 2.6.1.19, EC 2.6.1.22, GABA transaminase, GABA-AT, GABA-T, GABAT, L-AIBAT

Gene Symbol

ABAT

Additional ABAT Products

Product Documents for ABAT Antibody (OTI6C9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ABAT Antibody (OTI6C9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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