ABCF1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89314

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ABCF1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KKDVDDDGEEKELMERLKKLSVPTSDEEDEVPAPKPRGGKKTKGGNVFAALIQDQSEEEEEEEKHPPKPAKPEKNRINKAVSEEQQPALKGKKGKEEKSKGKAKPQNKFAAL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ABCF1 Antibody - BSA Free

Western Blot: ABCF1 Antibody [NBP1-89314]

Western Blot: ABCF1 Antibody [NBP1-89314]

Western Blot: ABCF1 Antibody [NBP1-89314] - Western blot analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: ABCF1 Antibody [NBP1-89314]

Immunocytochemistry/ Immunofluorescence: ABCF1 Antibody [NBP1-89314]

Immunocytochemistry/Immunofluorescence: ABCF1 Antibody [NBP1-89314] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314]

Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314]

Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Western Blot: ABCF1 Antibody [NBP1-89314]

Western Blot: ABCF1 Antibody [NBP1-89314]

Western Blot: ABCF1 Antibody [NBP1-89314] - Analysis in human cell lines Caco-2 and MCF-7 using anti-ABCF1 antibody. Corresponding ABCF1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314]

Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314]

Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314]

Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314]

Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314] - Staining of human pancreas shows weak to moderate cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314]

Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314]

Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314] - Staining of human rectum shows moderate to strong cytoplasmic positivity in glandular cells.

Applications for ABCF1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ABCF1

ABCF1 is encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process. [provided by RefSeq]

Alternate Names

ABC27, ABC50TNFalpha-inducible ATP-binding protein, ATP-binding cassette 50, ATP-binding cassette 50 (TNF-alpha stimulated), ATP-binding cassette sub-family F member 1, ATP-binding cassette, sub-family F (GCN20), member 1, EC 2.7.7.6, EC 3.6.3.17, EST123147, TNF-alpha-stimulated ABC protein

Gene Symbol

ABCF1

Additional ABCF1 Products

Product Documents for ABCF1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ABCF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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