Abraxas Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-22978
![Immunohistochemistry: Abraxas Antibody [NBP1-22978]](https://resources.rndsystems.com/images/products/Abraxas-Antibody-Immunohistochemistry-NBP1-22978-img0005.jpg "Immunohistochemistry: Abraxas Antibody [NBP1-22978]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 400 and 409 of human Abraxas (coiled-coil domain containing 98) using the numbering given in entry NP_620775.2 (GeneID 84142).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Abraxas Antibody - BSA Free
Immunohistochemistry: Abraxas Antibody [NBP1-22978]
Immunohistochemistry: Abraxas Antibody [NBP1-22978] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Abraxas used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.![Immunohistochemistry: Abraxas Antibody [NBP1-22978]](https://resources.rndsystems.com/images/products/Abraxas-Antibody-Immunohistochemistry-NBP1-22978-img0004.jpg "Immunohistochemistry: Abraxas Antibody [NBP1-22978]")
Immunohistochemistry: Abraxas Antibody [NBP1-22978]
Immunohistochemistry: Abraxas Antibody [NBP1-22978] - Sample: FFPE section of human ovarian carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit![Immunoprecipitation: Abraxas Antibody [NBP1-22978]](https://resources.rndsystems.com/images/products/Abraxas-Antibody-Immunoprecipitation-NBP1-22978-img0003.jpg "Immunoprecipitation: Abraxas Antibody [NBP1-22978]")
Immunoprecipitation: Abraxas Antibody [NBP1-22978]
Immunoprecipitation: Abraxas Antibody [NBP1-22978] - Whole cell lysate from HeLa cells. Affinity purified rabbit anti-Abraxas antibody used for IP at 10 mcg/mg lysate. Antibody was also immunoprecipitated by rabbit anti-Abraxas antibody NBP1-22977.Applications for Abraxas Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
5-15 ug/mg lysate
Application Notes
It is not recommended for western analysis.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Abraxas
Alternate Names
ABRA1, BRCA1-A complex subunit Abraxas, CCDC98, family with sequence similarity 175, member A
Entrez Gene IDs
84142 (Human)
Gene Symbol
ABRAXAS1
UniProt
Additional Abraxas Products
Product Documents for Abraxas Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Abraxas Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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