Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ABT1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1 to 50 of human Activator of Basal Transcription 1 using the numbering given in entry NP_037507.1 (GeneID 29777).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ABT1 Antibody - BSA Free

Western Blot: ABT1 Antibody [NBP2-22332]

Western Blot: ABT1 Antibody [NBP2-22332]

Western Blot: ABT1 Antibody [NBP2-22332] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22332 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: ABT1 Antibody [NBP2-22332]

Immunoprecipitation: ABT1 Antibody [NBP2-22332]

Immunoprecipitation: ABT1 Antibody [NBP2-22332] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22332 used for IP at 6 ug/mg lysate. ABT1 was also immunoprecipitated by rabbit anti-ABT1 antibody. For blotting immunoprecipitated ABT1, NBP2-22332 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for ABT1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ABT1

Basal transcription of genes by RNA polymerase II requires the interaction of TATA-binding protein (TBP) with the core region of class II promoters. Studies in mouse suggest that the protein encoded by this gene likely activates basal transcription from class II promoters by interaction with TBP and the class II promoter DNA.

Alternate Names

activator of basal transcription 1, Basal transcriptional activator, hABT1, TATA-binding protein-binding protein

Gene Symbol

ABT1

UniProt

NP_037507.1 (Human)

Additional ABT1 Products

Product Documents for ABT1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ABT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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