ACADS Antibody (OTI1D2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70061

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D2

Format

Azide and BSA Free
View all ACADS Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 34-260 of human ACADS (NP_000008) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

41.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACADS Antibody (OTI1D2) - Azide and BSA Free

Immunohistochemistry: ACADS Antibody (OTI1D2) - Azide and BSA Free [NBP2-70061]

Immunohistochemistry: ACADS Antibody (OTI1D2) - Azide and BSA Free [NBP2-70061]

Immunohistochemistry: ACADS Antibody (OTI1D2) - Azide and BSA Free [NBP2-70061] - Analysis of Human liver tissue.
Western Blot: ACADS Antibody (OTI1D2)Azide and BSA Free [NBP2-70061]

Western Blot: ACADS Antibody (OTI1D2)Azide and BSA Free [NBP2-70061]

Western Blot: ACADS Antibody (OTI1D2) - Azide and BSA Free [NBP2-70061] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western Blot: ACADS Antibody (OTI1D2)Azide and BSA Free [NBP2-70061]

Western Blot: ACADS Antibody (OTI1D2)Azide and BSA Free [NBP2-70061]

Western Blot: ACADS Antibody (OTI1D2) - Azide and BSA Free [NBP2-70061] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACADS.
Immunohistochemistry: ACADS Antibody (OTI1D2) - Azide and BSA Free [NBP2-70061]

Immunohistochemistry: ACADS Antibody (OTI1D2) - Azide and BSA Free [NBP2-70061]

Immunohistochemistry: ACADS Antibody (OTI1D2) - Azide and BSA Free [NBP2-70061] - Analysis of Human pancreas tissue.

Applications for ACADS Antibody (OTI1D2) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ACADS

ACADS encodes a a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with Short Chain Acyl-CoA Dehydrogenase Deficiency. [provided by RefSeq]

Alternate Names

ACAD3, acyl-CoA dehydrogenase, C-2 to C-3 short chain, acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain, Butyryl-CoA dehydrogenase, EC 1.3.99, EC 1.3.99.2, mitochondrial short-chain specific acyl-CoA dehydrogenase, SCADshort-chain acyl-CoA dehydrogenase, short-chain specific acyl-CoA dehydrogenase, mitochondrial, unsaturated acyl-CoA reductase

Gene Symbol

ACADS

Additional ACADS Products

Product Documents for ACADS Antibody (OTI1D2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ACADS Antibody (OTI1D2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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