ACADVL Antibody

Novus Biologicals | Catalog # NBP2-15238

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (91%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all ACADVL Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACADVL. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (81%).

Localization

Mitochondrion inner membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACADVL Antibody

Immunocytochemistry/ Immunofluorescence: ACADVL Antibody [NBP2-15238]

Immunocytochemistry/ Immunofluorescence: ACADVL Antibody [NBP2-15238]

Immunocytochemistry/Immunofluorescence: ACADVL Antibody [NBP2-15238] - ACADVL antibody detects ACADVL protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ACADVL stained by ACADVL antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.
Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238]

Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238]

Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238] - Rat liver. ACADVL stained by ACADVL antibody [N1C1] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: ACADVL Antibody [NBP2-15238]

Western Blot: ACADVL Antibody [NBP2-15238]

Western Blot: ACADVL Antibody [NBP2-15238] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE, antibody diluted at 1:3000.
Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238]

Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238]

Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238] - H358 xenograft, using ACADVL antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238]

Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238]

Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238] - Human A549 xenograft. ACADVL antibody [N1C1] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238]

Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238]

Immunohistochemistry-Paraffin: ACADVL Antibody [NBP2-15238] - Mouse kidney. ACADVL stained by ACADVL antibody [N1C1] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
ACADVL Antibody

Western Blot: ACADVL Antibody [NBP2-15238] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody [N1C1] (NBP2-15238) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
ACADVL Antibody

Western Blot: ACADVL Antibody [NBP2-15238] -

Wild-type (WT) and ACADVL knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody [N1C1] (NBP2-15238) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ACADVL Antibody

Western Blot: ACADVL Antibody [NBP2-15238] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody [N1C1] (NBP2-15238) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for ACADVL Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ACADVL

The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Alternate Names

ACAD6, acyl-CoA dehydrogenase, very long chain, acyl-Coenzyme A dehydrogenase, very long chain, EC 1.3.99, EC 1.3.99.-, LCACD, very long-chain specific acyl-CoA dehydrogenase, mitochondrial, VLCAD

Gene Symbol

ACADVL

UniProt

P49748 (Human)

Additional ACADVL Products

Product Documents for ACADVL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ACADVL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for ACADVL Antibody

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Protocols

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FAQs

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