ACD Antibody (OTI1A2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-72170

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A2

Format

Azide and BSA Free
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Product Specifications

Immunogen

Full length human recombinant protein of human ACD(NP_001075955) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

57.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse ACD Antibody (OTI1A2) - Azide and BSA Free (NBP2-01851) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ACD Antibody (OTI1A2) - Azide and BSA Free

Western Blot: ACD Antibody (OTI1A2)Azide and BSA Free [NBP2-72170]

Western Blot: ACD Antibody (OTI1A2)Azide and BSA Free [NBP2-72170]

Western Blot: ACD Antibody (OTI1A2) - Azide and BSA Free [NBP2-72170] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACD.
Immunocytochemistry/ Immunofluorescence: ACD Antibody (OTI1A2) - Azide and BSA Free [NBP2-72170]

Immunocytochemistry/ Immunofluorescence: ACD Antibody (OTI1A2) - Azide and BSA Free [NBP2-72170]

Immunocytochemistry/Immunofluorescence: ACD Antibody (OTI1A2) - Azide and BSA Free [NBP2-72170] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ACD.
Flow Cytometry: ACD Antibody (OTI1A2) - Azide and BSA Free [NBP2-72170]

Flow Cytometry: ACD Antibody (OTI1A2) - Azide and BSA Free [NBP2-72170]

Flow Cytometry: ACD Antibody (OTI1A2) - Azide and BSA Free [NBP2-72170] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-ACD antibody, and then analyzed by flow cytometry.

Applications for ACD Antibody (OTI1A2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ACD

ACD encodes a protein that is involved in telomere function. This protein is one of six core proteins in the telosome/shelterin telomeric complex, which functions to maintain telomere length and to protect telomere ends. Through its interaction with other components, this protein plays a key role in the assembly and stabilization of this complex, and it mediates the access of telomerase to the telomere. Multiple transcript variants encoding different isoforms have been found for this gene. This gene, which is also referred to as TPP1, is distinct from the unrelated TPP1 gene on chromosome 11, which encodes tripeptidyl-peptidase I.

Alternate Names

adrenocortical dysplasia homolog (mouse), Pip1, PIP1Tint1, POT1 and TIN2 organizing protein, POT1 and TIN2-interacting protein, Ptop, PTOPTpp1, TIN2 interacting protein 1, TINT1adrenocortical dysplasia protein homolog, TPP1

Gene Symbol

ACD

Additional ACD Products

Product Documents for ACD Antibody (OTI1A2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ACD Antibody (OTI1A2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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