Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-05407

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Acetyl-CoA Carboxylase alpha/ACACA Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S79 of human Acetyl-CoA Carboxylase alpha/ACACA (NP_942133.1). SMSGL

Modification

p Ser79

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] AntibodyAzide and BSA Free [NBP3-05407]

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] AntibodyAzide and BSA Free [NBP3-05407]

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [ac Ser79] Antibody [NBP3-05407] - Western blot analysis of extracts of 293T cells, using Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-05407) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide and BSA Free

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide and BSA Free [Acetyl-CoA Carboxylase alpha/ACACA] -

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide and BSA Free [Acetyl-CoA Carboxylase alpha/ACACA] - Western blot analysis of lysates from RAW264.7 cells, using Acetyl-CoA Carboxylase alpha/ACACA Rabbit pAb at 1:700 dilution. RAW264. 7 cells were treated by lambda-PP mixed solution (1ul) at 30C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide and BSA Free

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide and BSA Free [Acetyl-CoA Carboxylase alpha/ACACA] -

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide and BSA Free [Acetyl-CoA Carboxylase alpha/ACACA] - Western blot analysis of lysates from A-431 cells, using Acetyl-CoA Carboxylase alpha/ACACA Rabbit pAb at 1:700 dilution. A-431 cells were treated by lambda-PP mixed solution (1ul) at 30C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

Applications

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Acetyl-CoA Carboxylase alpha/ACACA

Acetyl-CoA carboxylase 1 (ACC1) is a biotin dependent lipogenic enzyme that is highly expressed during adipogenesis. ACC1 catalyzes acetyl-CoA carboxylation, producing malonyl-CoA, a metabolite involved in energy homeostasis regulation. Malonyl-CoA is a two carbon donor in the synthesis of long-chain fatty acids and the elongation of fatty acids found in the cystol (1). ACC1 is regulated short-term by citrate, CoA, and palmitoyl-CoA through allosteric interactions. Nutrients and hormones can be both short-term (inducing reversible phosphorylations by such as AMPK) and long-term (transcription level regulation) regulators of ACC1 (2). Highly expressed in lipogenic tissues, ACC1 is found in liver, adipose, and lactating mammary gland (3). ACC1 has been implicated as a target in the development of anti-obesity drugs (4).

Long Name

Acetyl-Coenzyme A Carboxylase alpha

Alternate Names

ACACA, ACC1, ACCA, AcetylCoA Carboxylase alpha

Gene Symbol

ACACA

Additional Acetyl-CoA Carboxylase alpha/ACACA Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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