Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide Free

Novus Biologicals | Catalog # NBP3-13324

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Porcine (100%), Rhesus Macaque (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all Acetyl-CoA Carboxylase alpha/ACACA Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser79 of rat Acetyl-CoA Carboxylase alpha/ACACA. The exact sequence is proprietary.

Modification

p Ser79

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

265 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide Free

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody [NBP3-13324]

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody [NBP3-13324]

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody [NBP3-13324] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Acetyl-CoA Carboxylase alpha/ACACA (phospho Ser79) antibody (NBP3-13324) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody [NBP3-13324]

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody [NBP3-13324]

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody [NBP3-13324] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Acetyl-CoA Carboxylase alpha/ACACA (phospho Ser79) antibody (NBP3-13324) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody [NBP3-13324] -

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody [NBP3-13324] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-13324) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide Free

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody [NBP3-13324] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Acetyl-CoA Carboxylase 1 (phospho Ser79) antibody (NBP3-13324) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

Azide Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Acetyl-CoA Carboxylase alpha/ACACA

Acetyl-CoA carboxylase 1 (ACC1) is a biotin dependent lipogenic enzyme that is highly expressed during adipogenesis. ACC1 catalyzes acetyl-CoA carboxylation, producing malonyl-CoA, a metabolite involved in energy homeostasis regulation. Malonyl-CoA is a two carbon donor in the synthesis of long-chain fatty acids and the elongation of fatty acids found in the cystol (1). ACC1 is regulated short-term by citrate, CoA, and palmitoyl-CoA through allosteric interactions. Nutrients and hormones can be both short-term (inducing reversible phosphorylations by such as AMPK) and long-term (transcription level regulation) regulators of ACC1 (2). Highly expressed in lipogenic tissues, ACC1 is found in liver, adipose, and lactating mammary gland (3). ACC1 has been implicated as a target in the development of anti-obesity drugs (4).

Long Name

Acetyl-Coenzyme A Carboxylase alpha

Alternate Names

ACACA, ACC1, ACCA, AcetylCoA Carboxylase alpha

Gene Symbol

ACACA

Additional Acetyl-CoA Carboxylase alpha/ACACA Products

Product Documents for Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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