ACSL1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89271

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ACSL1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YATRPKPLKPPCDLSMQSVEVAGSGGARRSALLDSDEPLVYFYDDVTTLYEGFQRGIQVSNNGPCLGSRKPDQPYEWLSYKQVAELSE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ACSL1 Antibody - BSA Free

Western Blot: ACSL1 Antibody [NBP1-89271]

Western Blot: ACSL1 Antibody [NBP1-89271]

Western Blot: ACSL1 Antibody [NBP1-89271] - Analysis using Anti-ACSL1 antibody NBP1-89271 (A) shows similar pattern to independent antibody NBP1-89270 (B).
Immunocytochemistry/ Immunofluorescence: ACSL1 Antibody [NBP1-89271]

Immunocytochemistry/ Immunofluorescence: ACSL1 Antibody [NBP1-89271]

Immunocytochemistry/Immunofluorescence: ACSL1 Antibody [NBP1-89271] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271]

Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271]

Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Western Blot: ACSL1 Antibody [NBP1-89271]

Western Blot: ACSL1 Antibody [NBP1-89271]

Western Blot: ACSL1 Antibody [NBP1-89271] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271]

Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271]

Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human kidney, liver, soft tissues and tonsil using Anti-ACSL1 antibody NBP1-89271 (A) shows similar protein distribution across tissues to independent antibody NBP1-89270 (B).
Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271]

Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271]

Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Analysis in human liver and tonsil tissues. Corresponding ACSL1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271]

Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271]

Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human tonsil shows no cytoplasmic positivity in germinal center cells as expected.
Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271]

Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271]

Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human soft tissues shows strong cytoplasmic positivity in adipocytes.
Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271]

Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271]

Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271]

Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271]

Immunohistochemistry-Paraffin: ACSL1 Antibody [NBP1-89271] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.

Applications for ACSL1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACSL1

Long-chain-fatty-acid--CoA ligase 1, or ACSL1, is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. These proteins play an important role in lipid biosynthesis and fatty acid degradation. Like other isozymes of this family, ALCS1 converts free long-chain fatty acids into fatty acyl-CoA esters. ACSL1 preferentially uses palmitoleate, oleate and linoleate, and altered expression of ACSL1 is thought to increase accumulation of triglycerides in the liver.

ALCS1 is strongly expressed in the heart, kindey, liver, skeletal muscle, and erythroid cells, and to a lesser extent in lung, brain, placenta and pancreas. This protein is predominantly expressed during the early stages of erythroid development, and expression is weak in reticulocytes and young erythrocytes.

Alternate Names

ACS1LACS 2, Acyl-CoA synthetase 1, acyl-CoA synthetase long-chain family member 1, EC 6.2.1, FACL1EC 6.2.1.3, fatty-acid-Coenzyme A ligase, long-chain 1, LACS 1, LACSlong-chain 2, lignoceroyl-CoA synthase, Long-chain acyl-CoA synthetase 1, Long-chain acyl-CoA synthetase 2, Long-chain fatty acid-CoA ligase 2, long-chain fatty-acid-coenzyme A ligase 1, long-chain-fatty-acid--CoA ligase 1, Palmitoyl-CoA ligase 1, Palmitoyl-CoA ligase 2, paltimoyl-CoA ligase 1

Gene Symbol

ACSL1

Additional ACSL1 Products

Product Documents for ACSL1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ACSL1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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