Actin Gamma 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-46266

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Actin Gamma 1 Primary Antibodies »

Product Specifications

Immunogen

actin, gamma 1. (Uniprot# P63261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Actin Gamma 1 Antibody - BSA Free

Actin Gamma 1 Antibody - BSA Free

Western Blot: Actin Gamma 1 Antibody - BSA Free [NBP3-46266] -

Western Blot: Actin Gamma 1 Antibody - BSA Free [NBP3-46266] - HeLa cells were subjected to SDS PAGE followed by western blot with (Actin Gamma 1 antibody) at dilution of 1:500

Applications for Actin Gamma 1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% Glycerol, pH (7.3)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Actin Gamma 1

Actin, gamma 1 (ACTG1) is a member of the actin protein family, a highly conserved protein group with three main isoforms: alpha, beta and gamma. Alpha actins are mainly expressed in muscle tissues where they are a major constituent of the contractile apparatus, whereas beta and gamma actin proteins are widely expressed in most cell types where they are predominantly involved in cell motility and cytoskeleton maintenance. Actin gamma 1 is a cytoplasmic actin isoform usually found in nonmuscle cells.

Actin Gamma 1 antibodies serve as excellent loading controls, due to the proteins' near ubiquitous expressn and strong conservation across most species. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Actin Gamma 1 antibody serves as an excellent loading control.

Alternate Names

ACT, ACTB, ACTG, actin, cytoplasmic 2, actin, gamma 1, cytoskeletal gamma-actin, deafness, autosomal dominant 20; deafness, autosomal dominant 26, DFNA20, DFNA26, Gamma-actin

Gene Symbol

ACTG1

Additional Actin Gamma 1 Products

Product Documents for Actin Gamma 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Actin Gamma 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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