Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-48154

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Chicken, Feline, Rabbit

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HHF35

Format

IHC-Prediluted
View all Actin (Muscle Specific) Primary Antibodies »

Product Specifications

Immunogen

SDS extract of human myocardium.

Localization

Cytoplasmic

Marker

Muscle Cell Marker

Specificity

This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154] - Human Rhabdomyosarcoma stained with Muscle Specific Actin Mouse Monoclonal Antibody (HHF35).
Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154]

Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154] - Human Leiomyosarcoma stained with Muscle Specific Actin Mouse Monoclonal Antibody (HHF35).
Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted Western Blot: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154] -

Western Blot: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154] -

Western Blot Analysis of Endometrium muscle tissue lysate using Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted.
Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted Western Blot: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154] -

Western Blot: Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted [NBP2-48154] -

Western Blot Analysis of Skeletal muscle tissue lysate using Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted.

Applications for Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Immunohistochemistry (No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.).
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Actin (Muscle Specific)

Alternate Names

ACTA, ACTC, actin, alpha 2, smooth muscle, aorta, actin, alpha cardiac muscle 1, actin, alpha skeletal muscle, actin, aortic smooth muscle, ACTSA, alpha-cardiac actin, ASD5, ASMA, Cell growth-inhibiting gene 46 protein, CFTD, CFTD1, CFTDM, CMD1R, CMH11, MPFD, NEM1, NEM2, NEM3, nemaline myopathy type 3

Gene Symbol

ACTA1

UniProt

P68032, P68133 (Human), P62736 (Human)

Additional Actin (Muscle Specific) Products

Product Documents for Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted

Certificate of Analysis

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Product Specific Notices for Actin (Muscle Specific) Antibody (HHF35) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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