actin-related protein 2/3 complex subunit 1B Antibody
Novus Biologicals | Catalog # NBP1-46220
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Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 322 and 372 of human Actin Related Protein 2/3 complex, Subunit 1B using the numbering given in entry NP_005711.1 (GeneID 10095).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for actin-related protein 2/3 complex subunit 1B Antibody
Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-46220]
Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-46220] - Detection of Human and Mouse Arpc1b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Arpc1b antibody NBP1-46220 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Immunoprecipitation: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-46220]
Immunoprecipitation: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-46220] - Detection of human Arpc1b by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Arpc1b antibody NBP1-46220 (lot NBP1-46220-3) used for IP at 6 ug per reaction. Arpc1b was also immunoprecipitated by a previous lot of this antibody (lot NBP1-46220-2) and rabbit anti-Arpc1b antibody NBP1-46219. For blotting immunoprecipitated Arpc1b, NBP1-46220 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for actin-related protein 2/3 complex subunit 1B Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: actin-related protein 2/3 complex subunit 1B
Alternate Names
actin related protein 2/3 complex, subunit 1B (41 kD), actin related protein 2/3 complex, subunit 1B, 41kDa, actin-related protein 2/3 complex subunit 1B, ARC41p41-ARCp40-ARC, Arp2/3 complex 41 kDa subunit, ARP2/3 protein complex subunit p41
Gene Symbol
ARPC1B
Additional actin-related protein 2/3 complex subunit 1B Products
Product Documents for actin-related protein 2/3 complex subunit 1B Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for actin-related protein 2/3 complex subunit 1B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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