ACTL7B Antibody

Novus Biologicals | Catalog # NB100-57848

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Rat

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ACTL7B Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DELHVDYELPDGK corresponding to internal region according to NP_079547.1.

Specificity

This antibody is not expected to cross-react with Actin-like 7A.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACTL7B Antibody

Western Blot: ACTL7B Antibody [NB100-57848]

Western Blot: ACTL7B Antibody [NB100-57848]

Western Blot: ACTL7B Antibody [NB100-57848] - (0.5ug/ml) staining of Rat Testes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for ACTL7B Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 1.5 ug/ml
Application Notes
WB: Approx. 45 kDa band observed in rat testis lysates (calculated MW of 45.5 kDa band according to rat NP_001020588.1 and 45.6 kDa band according to mouse NP_079547.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACTL7B

The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene (ACTL7B), and related gene, ACTL7A, are intronless, and are located approximately 4 kb apart in a head-to-head orientation within the familial dysautonomia candidate region on 9q31. Based on mutational analysis of the ACTL7B gene in patients with this disorder, it was concluded that it is unlikely to be involved in the pathogenesis of dysautonomia. Unlike ACTL7A, the ACTL7B gene is expressed predominantly in the testis, however, its exact function is not known.

Alternate Names

actin-like 7B, actin-like 7-beta, actin-like protein 7B, Actin-like-7-beta, Tact1

Entrez Gene IDs

11471 (Mouse); 313183 (Rat)

Gene Symbol

ACTL7B

UniProt

Q4QR76 (Rat)

Additional ACTL7B Products

Product Documents for ACTL7B Antibody

Certificate of Analysis

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Product Specific Notices for ACTL7B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ACTL7B Antibody

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