Adenylosuccinate Synthase Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-92661
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ADSS (NP_001117.2). MAFAETYPAASSLPNGDCGRPRARPGGNRVTVVLGAQWGDEGKGKVVDLLAQDADIVCRCQGGNNAGHTVVVDSVEYDFHLLPSGIINPNVTAFIGNGVVIHLPGLFEEAEKNVQKGKGLEGWEKRLIISDRAHIVFDFHQAADGIQEQQRQEQAGKNLGTTKKGIGPVYSSKAARSGLRMCDLVSDFDGFSERFKVLANQYKSIYPTLEIDIEGELQKLKGYMEKIKPMVRDGVYFLYEALHGPPKKILVEGANAALLDIDFGTYPFVTSSNCTVGGVC
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Adenylosuccinate Synthase Antibody - BSA Free
Western Blot: Adenylosuccinate Synthase AntibodyBSA Free [NBP2-92661]
Western Blot: Adenylosuccinate Synthase Antibody [NBP2-92661] - Analysis of extracts of various cell lines, using Adenylosuccinate Synthase at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.Immunohistochemistry: Adenylosuccinate Synthase Antibody - BSA Free [NBP2-92661] -
Immunohistochemistry: Adenylosuccinate Synthase Antibody - BSA Free [NBP2-92661] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Adenylosuccinate Synthase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.Immunohistochemistry: Adenylosuccinate Synthase Antibody - BSA Free [NBP2-92661] -
Immunohistochemistry: Adenylosuccinate Synthase Antibody - BSA Free [NBP2-92661] - Immunohistochemistry analysis of paraffin-embedded Human colon using Adenylosuccinate Synthase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.Immunocytochemistry/ Immunofluorescence: Adenylosuccinate Synthase Antibody - BSA Free [NBP2-92661] -
Immunocytochemistry/ Immunofluorescence: Adenylosuccinate Synthase Antibody - BSA Free [NBP2-92661] - Immunofluorescence analysis of HeLa cells using Adenylosuccinate Synthase Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Immunohistochemistry: Adenylosuccinate Synthase Antibody - BSA Free [NBP2-92661] -
Immunohistochemistry: Adenylosuccinate Synthase Antibody - BSA Free [NBP2-92661] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using Adenylosuccinate Synthase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.Immunohistochemistry: Adenylosuccinate Synthase Antibody - BSA Free [NBP2-92661] -
Immunohistochemistry: Adenylosuccinate Synthase Antibody - BSA Free [NBP2-92661] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using Adenylosuccinate Synthase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.Western Blot: Adenylosuccinate Synthase Antibody - BSA Free [NBP2-92661] -
Western Blot: Adenylosuccinate Synthase Antibody - BSA Free [NBP2-92661] - Western blot analysis of various lysates using Adenylosuccinate Synthase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Applications for Adenylosuccinate Synthase Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Adenylosuccinate Synthase
Alternate Names
ADEH, adenylosuccinate synthase, adenylosuccinate synthetase (Ade(-)H-complementing), adenylosuccinate synthetase isozyme 2, Adenylosuccinate synthetase, acidic isozyme, Adenylosuccinate synthetase, liver isozyme, AdSS 2, ADSS2, AMPSase 2, EC 6.3.4.4, IMP--aspartate ligase 2, L-type adenylosuccinate synthetase, MGC20404
Gene Symbol
ADSS2
Additional Adenylosuccinate Synthase Products
Product Documents for Adenylosuccinate Synthase Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Adenylosuccinate Synthase Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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