AF9 Antibody

Novus Biologicals | Catalog # NBP2-15303

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation Sequencing

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all AF9 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human AF9. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AF9 Antibody

Immunocytochemistry/ Immunofluorescence: AF9 Antibody [NBP2-15303]

Immunocytochemistry/ Immunofluorescence: AF9 Antibody [NBP2-15303]

Immunocytochemistry/Immunofluorescence: AF9 Antibody [NBP2-15303] - AF9 antibody detects AF9 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AF9 protein stained by AF9 antibody diluted at 1:1000. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 um.
Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303]

Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303]

Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303] - Mouse liver. AF9 stained by AF9 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: AF9 Antibody [NBP2-15303]

Western Blot: AF9 Antibody [NBP2-15303]

Western Blot: AF9 Antibody [NBP2-15303] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody
Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303]

Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303]

Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303] - D54 xenograft, using AF9 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303]

Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303]

Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303] - Mouse brain. AF9 stained by AF9 antibody diluted at 1:1500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
AF9 Antibody

Western Blot: AF9 Antibody [NBP2-15303] -

Western Blot: AF9 Antibody [NBP2-15303] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody (NBP2-15303) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
AF9 Antibody

Western Blot: AF9 Antibody [NBP2-15303] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody (NBP2-15303) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for AF9 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation Sequencing

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AF9

The human AF9 gene is one of the most common fusion partner genes with the ALL1 gene at 11q23 (also called MLL), resulting in the t(9;11)(p22;q23) fusion. The AF9 gene is more than 100 kb, and 2 patient breakpoint cluster regions (BCRs) have been identified. BCR1 is found within intron 4, previously called site A, whereas BCR2 or site B spans introns 7 and 8.

Alternate Names

AF-9, AF9myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 3, ALL1-fused gene from chromosome 9 protein, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocatedto, 3, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 3, Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein, protein AF-9, YEATS domain-containing protein 3, YEATS3FLJ2035

Gene Symbol

MLLT3

UniProt

P42568 (Human)

Additional AF9 Products

Product Documents for AF9 Antibody

Certificate of Analysis

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Product Specific Notices for AF9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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