Q: My question was how sensitive is your most sensitive aflatoxin antibody? Say in a standard ELISA plate format. I could not find any info of that kind on your website.

A: We have one kit available, cat# KA1412, that can detect Aflatoxin levels of less than 1 ng/mL. The only other sensitivity information we have is that NB120-1017 can detect Aflatoxin when used at a concentration of 5ug/mL and NB100-2908 has a recommended dilution of 1:2000-1:10000 for use in ELISA.

Aflatoxin B1 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # KA1412

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Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Corns, feeds, cotton seed and peanuts (25 uL)

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Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Description

Quality control test: Standard curve. Aflatoxin B ELISA Kit is a competitive binding enzyme immunoassay for the quantitative measurement of aflatoxin B.

Scientific Data Images for Aflatoxin B1 ELISA Kit (Colorimetric)

ELISA: Aflatoxin B1 ELISA Kit (Colorimetric) [KA1412]

ELISA: Aflatoxin B1 ELISA Kit (Colorimetric) [KA1412] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Aflatoxin B1 ELISA Kit (Colorimetric)

96-wells microtiter plate (#S). Twelve strips of 8 detachable wells, coated with rabbit Anti-Aflatoxin antibody
Aflatoxin-Alkaline Phosphatase conjugate (AFX-ALP). (#3)
Calibrator containing 0, 0.5, 2.0 and 8.0 ng/mL of Aflatoxin B1
p-Nitrophenyl Phosphate (pNPP) substrate. Ready to use. (#5)
Stop Solution, 3 N NaOH. (#7)
Wash Buffer (10xPBS-Tween). Dilute 10 fold with distilled or deionized water to 150 mL prior to use. (#6)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Aflatoxin B1

Aflatoxin B1 is a naturally occurring mycotoxin produced by two types of mold: Aspergillus flavus and Aspergillus parasiticus. These molds commonly affect grains which are grown under stressful conditions such as drought and also grows on food that is stored in hot and humid conditions.

There are 13 known Aflatoxins produced, with aflatoxin B1 considered as the most toxic. In fact, Aflatoxin B1 is the most potent hepatocellular carcinoma (HCC) causing chemical currently known. Specifically, Aflatoxin B1 is thought to case mutations in the p53 gene which interfere with the gene's critical tumor suppressing functions.

Aflatoxin B1 are useful tools for studying hepatocellular carcinoma formation with wide ranging implications into public health and food saftey.

Alternate Names

AFB1, AFB1-AR1, Aldoketoreductase 7

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Product Documents for Aflatoxin B1 ELISA Kit (Colorimetric)

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Certificate of Analysis

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Product Specific Notices for Aflatoxin B1 ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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