AIF-1/Iba1 Antibody (CL10936) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44312

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL10936

Format

Azide and BSA Free
View all AIF-1/Iba1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P55008, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for AIF-1/Iba1 Antibody (CL10936) - Azide and BSA Free

AIF-1/Iba1 Antibody (CL10936) - Azide and BSA Free Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP3-44312]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP3-44312]

Staining of human cerebellum shows moderate cytoplasmic positivity in microglia.
AIF-1/Iba1 Antibody (CL10936) - Azide and BSA Free Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP3-44312]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP3-44312]

Staining of human cerebral cortex shows moderate cytoplasmic positivity in microglia.
AIF-1/Iba1 Antibody (CL10936) - Azide and BSA Free Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP3-44312]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP3-44312]

Staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.
AIF-1/Iba1 Antibody (CL10936) - Azide and BSA Free Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP3-44312]

Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP3-44312]

Staining of human uterine cervix shows no positivity in squamous epithelial cells as expected.

Applications for AIF-1/Iba1 Antibody (CL10936) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AIF-1/Iba1

Allograft inflammatory factor (AIF-1) or ionized calcium-binding adapter molecule 1 (Iba1) is a cytosolic actin binding protein containing a calcium binding domain (EF-hand) and inducible by cytokines and IFN-gamma (1). AIF-1 (theoretical molecular weight 17kDa) was first cloned from rat and human cardiac allografts and macrophage cell lines. In cardiac allografts, expression of AIF-1 was specifically associated with infiltrated mononuclear cells (2, 3). AIF-1 is constitutively expressed and inducible in macrophages and microglia. Iba1, microglia response factor (MRF-1) and daintain were independently cloned and identified in rat and human tissues and share complete sequence identity with AIF-1. Additionally, several AIF-1 splice variants have been identified including IRT-1, BART-1, G1 and Hara-1 (2).

Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.

References

1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA. https://doi.org/10.1002/glia.10149

2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters. https://doi.org/10.1016/S0014-5793(02)02430-4

3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation. https://doi.org/10.1097/00007890-199605150-00018

4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells. https://doi.org/10.3390/cells8060639

5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T.,... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.5.3316

Long Name

Allograft Inflammatory Factor 1

Alternate Names

AIF1, IBA1, IRT1

Gene Symbol

AIF1

Additional AIF-1/Iba1 Products

Product Documents for AIF-1/Iba1 Antibody (CL10936) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for AIF-1/Iba1 Antibody (CL10936) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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