AKAP12 Antibody

Novus Biologicals | Catalog # NB100-68211

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all AKAP12 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1650 and 1700 of human A kinase (PRKA) anchor protein (gravin) 12 using the numbering given in entry AAC51366.1 (GeneID 9590).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AKAP12 Antibody

Western Blot: AKAP12 Antibody [NB100-68211]

Western Blot: AKAP12 Antibody [NB100-68211]

Western Blot: AKAP12 Antibody [NB100-68211] - Detection of Human AKAP12/Gravin on HeLa whole cell lysate using NB100-68211. AKAP12/Gravin was also immunoprecipitated by rabbit anti-AKAP12/Gravin antibody NB100-68212.

Applications for AKAP12 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: AKAP12

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Alternate Names

A kinase (PRKA) anchor protein (gravin) 12, A kinase (PRKA) anchor protein 12, AKAP 250, AKAP250250kDa, DKFZp686M0430, DKFZp686O0331, FLJ20945, FLJ97621, Gravin, kinase scaffold protein gravin, Myasthenia gravis autoantigen, myasthenia gravis autoantigen gravin

Entrez Gene IDs

9590 (Human)

Gene Symbol

AKAP12

UniProt

Q02952 (Human)

Additional AKAP12 Products

Product Documents for AKAP12 Antibody

Certificate of Analysis

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Product Specific Notices for AKAP12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for AKAP12 Antibody

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