alcohol dehydrogenase 5 Antibody

Novus Biologicals | Catalog # NB100-57101

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all alcohol dehydrogenase 5 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KKIKVDEFVTHN corresponding to internal region according to NP_000662.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alcohol dehydrogenase 5 Antibody

Western Blot: alcohol dehydrogenase 5 Antibody [NB100-57101]

Western Blot: alcohol dehydrogenase 5 Antibody [NB100-57101]

Western Blot: alcohol dehydrogenase 5 Antibody [NB100-57101] - (0.5ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for alcohol dehydrogenase 5 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000

Western Blot

0.5 - 1.5 ug/ml
Application Notes
WB: Approx. 38 kDa band observed in human testis lysates (calculated MW of 39.7 kDa band according to NP_000662.3). An anonymous customer has reported positive results on rat penis tissue and mouse penile tissue. IHC: An anonymous customer has reported positive results in on rat penile tissue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alcohol dehydrogenase 5

The ADH5 gene encodes glutathione dependent formaldehyde dehydrogenase or class III alcohol dehydrogenase chi subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, retinol, ethanol and other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class III alcohol dehydrogenase is a homodimer composed of 2 chi subunits. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long chain primary alcohols and for oxidation of S hydroxymethyl glutathione, a spontaneous adduct between formaldehyde and glutathione. The enzyme is an important component of cellular metabolism for the elimination of formaldehyde, which is a potent irritant and sensitizing agent that causes rhinitis, lacrymation, contact dermatitis and pharyngitis.

Alternate Names

ADH-3, ADHXEC 1.1.1.1, alcohol dehydrogenase (class III), chi polypeptide, Alcohol dehydrogenase 5, alcohol dehydrogenase 5 (class III), chi polypeptide, Alcohol dehydrogenase class chi chain, alcohol dehydrogenase class-3, Alcohol dehydrogenase class-III, EC 1.1.1, EC 1.1.1.-, EC 1.1.1.284, FALDH, FDHGSNOR, formaldehyde dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GSH-FDH, S-(hydroxymethyl)glutathione dehydrogenase

Entrez Gene IDs

128 (Human); 11532 (Mouse)

Gene Symbol

ADH5

UniProt

P11766 (Human)

Additional alcohol dehydrogenase 5 Products

Product Documents for alcohol dehydrogenase 5 Antibody

Certificate of Analysis

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Product Specific Notices for alcohol dehydrogenase 5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for alcohol dehydrogenase 5 Antibody

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