ALDH4A1 Antibody (002) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-90152

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 002 expressed in HEK293

Format

Azide and BSA Free
View all ALDH4A1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human ALDH4A1 (Accession#: AAH07581.1; Lys25-Gln563).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ALDH4A1 Antibody (002) - Azide and BSA Free

Western Blot: ALDH4A1 Antibody (002) [NBP2-90152]

Western Blot: ALDH4A1 Antibody (002) [NBP2-90152]

Western Blot: ALDH4A1 Antibody (002) [NBP2-90152] - Anti-ALDH4A1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L(Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 62 kDa Observed band size: 62 kDa (We are unsure as to the identity of these extra bands.)
Immunoprecipitation: ALDH4A1 Antibody (002) [NBP2-90152]

Immunoprecipitation: ALDH4A1 Antibody (002) [NBP2-90152]

Immunoprecipitation: ALDH4A1 Antibody (002) [NBP2-90152] - ALDH4A1 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate. Lane B:0.5 mg K562 Whole Cell Lysate. 2 uL anti-ALDH4A1 rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-ALDH4A1 rabbit monoclonal antibody,at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa.

Applications for ALDH4A1 Antibody (002) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALDH4A1

ALDH4A1 belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Two transcript variants encoding the same protein have been identified for this gene.

Alternate Names

aldehyde dehydrogenase 4 family, member A1, Aldehyde dehydrogenase family 4 member A1, ALDH4DKFZp779M035, delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial, EC 1.5.1.12, mitochondrial delta-1-pyrroline 5-carboxylate dehydrogenase, P5C dehydrogenase, P5CD, P5CDH

Gene Symbol

ALDH4A1

Additional ALDH4A1 Products

Product Documents for ALDH4A1 Antibody (002) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ALDH4A1 Antibody (002) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ALDH4A1 Antibody (002) - Azide and BSA Free

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