Aldo-keto Reductase 1B10/AKR1B10 Antibody

Novus Biologicals | Catalog # NB100-93370

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Aldo-keto Reductase 1B10/AKR1B10 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QSSHLEDYPFDAE corresponding to C-Terminus according to NP_064695.2.

Epitope

C-QSSHLEDYPFDAE

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Aldo-keto Reductase 1B10/AKR1B10 Antibody

Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370]

Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370]

Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370] - HEK293 overexpressing AKR1B10 (RC203177) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with NB100-93370 in the right panel (mock transfection in first and last lanes).
Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370]

Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370]

Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370] - Analysis of AKR1B10 using this antibody at 0.03 ug/ml in A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Aldo-keto Reductase 1B10/AKR1B10 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in lysates of HEK293, A549 and HepG2 (calculated MW of 36.0 kDa band according to NP_064695.2). In transfected HEK293 transiently expressing AKR1B10 a band of approx. 40 kDa is observed. This band is not observed in the non-transfected HEK293.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aldo-keto Reductase 1B10/AKR1B10

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq]

Long Name

Aldo-keto Reductase Family 1, Member B10 [Aldose Reductase]

Alternate Names

AKR1B10, AKR1B11, AKR1B12, AldoketoReductase 1B10, ARL1, HIS, HSI

Entrez Gene IDs

57016 (Human)

Gene Symbol

AKR1B10

UniProt

NP_064695.2 (Human)

Additional Aldo-keto Reductase 1B10/AKR1B10 Products

Product Documents for Aldo-keto Reductase 1B10/AKR1B10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Aldo-keto Reductase 1B10/AKR1B10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

Citations for Aldo-keto Reductase 1B10/AKR1B10 Antibody

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