Alkaline Phosphatase/ALPP Antibody (ALPP/9109R) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-24277

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Kappa Clone # ALPP/9109R

Format

Azide and BSA Free
View all Alkaline Phosphatase/ALPP Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Alkaline Phosphatase/ALPP protein

Localization

Cytoplasm. Cell surface.

Marker

Germ Cell Tumor Marker

Specificity

Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Antibody without azide - store at -20 to -80C.

Scientific Data Images for Alkaline Phosphatase/ALPP Antibody (ALPP/9109R) - Azide and BSA Free

Alkaline Phosphatase/ALPP Antibody (ALPP/9109R) - Azide and BSA Free Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (ALPP/9109R) - Azide and BSA Free [NBP3-24277] -

Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (ALPP/9109R) - Azide and BSA Free [NBP3-24277] -

Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (ALPP/9109R) - Azide and BSA Free [NBP3-24277] - Formalin-fixed, paraffin-embedded human tonsil stained with Alkaline Phosphatase/ALPP Antibody (ALPP/9109R). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for Alkaline Phosphatase/ALPP Antibody (ALPP/9109R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase/ALPP

Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer.

Long Name

Alkaline Phosphatase, Placental Type

Alternate Names

Alkaline Phosphatase, PLAP, SEAP

Gene Symbol

ALPP

Additional Alkaline Phosphatase/ALPP Products

Product Documents for Alkaline Phosphatase/ALPP Antibody (ALPP/9109R) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Alkaline Phosphatase/ALPP Antibody (ALPP/9109R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Phosphatases and Regulators

Customer Reviews for Alkaline Phosphatase/ALPP Antibody (ALPP/9109R) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Alkaline Phosphatase/ALPP Antibody (ALPP/9109R) - Azide and BSA Free and earn rewards!

Have you used Alkaline Phosphatase/ALPP Antibody (ALPP/9109R) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Alkaline Phosphatase/ALPP Antibody (ALPP/9109R) - Azide and BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?

    A:

    None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies