Alkaline Phosphatase, Intestinal Antibody (3A8) - Azide and BSA Free

Novus Biologicals | Catalog # H00000248-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3A8

Format

Azide and BSA Free
View all Alkaline Phosphatase, Intestinal Primary Antibodies »

Product Specifications

Immunogen

ALPI (NP_001622, 74 a.a. ~ 162 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LKGQKNGKLGPETPLAMDRFPYLALSKTYNVDRQVPDSAATATAYLCGVKANFQTIGLSAAARFNQCNTTRGNEVISVMNRAKQAGKSV

Specificity

ALPI - alkaline phosphatase, intestinal

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Alkaline Phosphatase, Intestinal Antibody (3A8) - Azide and BSA Free

Western Blot: Alkaline Phosphatase, Intestinal Antibody (3A8) [H00000248-M03]

Western Blot: Alkaline Phosphatase, Intestinal Antibody (3A8) [H00000248-M03]

Western Blot: Alkaline Phosphatase, Intestinal Antibody (3A8) [H00000248-M03] - Analysis of ALPI expression in HeLa (Cat # L013V1).
ELISA: Alkaline Phosphatase, Intestinal Antibody (3A8) [H00000248-M03]

ELISA: Alkaline Phosphatase, Intestinal Antibody (3A8) [H00000248-M03]

ELISA: Alkaline Phosphatase, Intestinal Antibody (3A8) [H00000248-M03] - Detection limit for recombinant GST tagged ALPI is 0.3 ng/ml as a capture antibody.

Applications for Alkaline Phosphatase, Intestinal Antibody (3A8) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase, Intestinal

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.

Long Name

Alkaline Phosphatase, Intestinal Type

Alternate Names

alkaline phosphatase, intestinal, Alkaline phosphomonoesterase, ALPI, EC 3.1.3.1, Glycerophosphatase, IAP, Intestinal alkaline phosphatase, intestinal-type alkaline phosphatase, Kasahara isozyme

Entrez Gene IDs

248 (Human)

Gene Symbol

ALPI

OMIM

171740 (Human)

UniProt

NP_001622 (Human)

Additional Alkaline Phosphatase, Intestinal Products

Product Documents for Alkaline Phosphatase, Intestinal Antibody (3A8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Alkaline Phosphatase, Intestinal Antibody (3A8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Alkaline Phosphatase, Intestinal Antibody (3A8) - Azide and BSA Free

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