Alkaline Phosphatase, Intestinal Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB120-7328

Novus Biologicals

Key Product Details

Species Reactivity

Bovine

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

Azide and BSA Free
View all Alkaline Phosphatase, Intestinal Primary Antibodies »

Product Specifications

Immunogen

Alkaline Phosphatase, Intestinal, Intestinal [Calf Intestine] (Uniprot: P19111)

Reactivity Notes

Cross reactivity against Alkaline Phosphatase, Intestinal from other tissues and species may occur but have not been specifically determined.

Localization

Attached to the membrane by a GPI-anchor.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Applications for Alkaline Phosphatase, Intestinal Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:4000-1:20000

Western Blot

1:500-1:2000
Application Notes
This product has been assayed against 1.0 ug of Alkaline Phosphatase [Calf intestine] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Alkaline Phosphatase, Intestinal

Alkaline phosphatase removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes - placental, placental-like, intestinal and non-tissue specific (found in liver, kidney and bone).

Long Name

Alkaline Phosphatase, Intestinal Type

Alternate Names

alkaline phosphatase, intestinal, Alkaline phosphomonoesterase, ALPI, EC 3.1.3.1, Glycerophosphatase, IAP, Intestinal alkaline phosphatase, intestinal-type alkaline phosphatase, Kasahara isozyme

Entrez Gene IDs

248 (Human)

Gene Symbol

ALPI

UniProt

P19111 (Bovine)

Additional Alkaline Phosphatase, Intestinal Products

Product Documents for Alkaline Phosphatase, Intestinal Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Alkaline Phosphatase, Intestinal Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Alkaline Phosphatase, Intestinal Antibody - Azide and BSA Free

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  • Q: I recently received the Alkaline Phosphatase (calf intestinal) antibody from Novus (Cat# NB120-7328, Lot# 170) and in initial experiments it works nicely. However, I would like to use it for quantitative experiments. Therefore, it would be very helpful if you could tell me the molar concentration of this antibody.

    A: The concentration of Lot 170 is 1mg/ml.

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