Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00)

Novus Biologicals | Catalog # NBP2-67295

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SA40-00 expressed in HEK293
View all Alkaline Phosphatase, Tissue Non-Specific Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human Alkaline Phosphatase, Tissue Non-Specific aa 18-50. (SwissProt: P05186 Human; SwissProt: P09242 Mouse; SwissProt: P08289 Rat)

Localization

Cell membrane, Mitochondrion membrane, Mitochondrion intermembrane space, Extracellular vesicle membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00)

Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295]

Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295]

Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Staining ALP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295]

Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295]

Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Analysis of Hela cells with ALP antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295]

Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295]

Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Staining ALP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Alkaline Phosphatase, Tissue Non-Specific antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The t
Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Alkaline Phosphatase, Tissue Non-Specific antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minut
Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Alkaline Phosphatase, Tissue Non-Specific antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The t
Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00)

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] -

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] -Analysis of Alkaline Phosphatase on different lysates with Rabbit anti-Alkaline Phosphatase antibody at 1/5,000 dilution.Lane 1: Saos-2 cell lysate (15ug/Lane)Lane 2: HeLa cell lysate (15 ug/Lane)Lane 3: A549 cell lysate (15 ug/Lane)Lane 4: Mouse liver tissue lysate (20 ug/Lane)Lane 5: Rat liver tissue lysate (20 ug/Lane)Predicted band size: 57 kDaObserved band size: 75 kDaExposure time: 24 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.

Applications for Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

Use at an assay dependent concentration

Western Blot

1:5,000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase, Tissue Non-Specific

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Alternate Names

Alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, liver/bone/kidney, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphomonoesterase, APTNAP, AP-TNAP, EC 3.1.3.1, FLJ40094, FLJ93059, glycerophosphatase, HOPS, liver/bone/kidney-type alkaline phosphatase, MGC161443, tissue-nonspecific ALP, TNAP, TNSALPMGC167935

Gene Symbol

ALPL

Additional Alkaline Phosphatase, Tissue Non-Specific Products

Product Documents for Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00)

Certificate of Analysis

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Product Specific Notices for Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00)

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Protocols

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