Alkaline Phosphatase, Tissue Non-Specific Antibody - BSA Free

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-32948] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody (NBP1-32948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody - BSA Free [NBP1-32948] -

Deprivation of NO induces bi-lineage phenotype and expression of stem cell markers in mammary epithelial cells both in vivo and in 3D cultures. (A) CK14 (basal) and CK8/18 (luminal) staining of mouse mammary glands treated with control (PBS), L-arginine or L-NAME for 6 weeks. Co-localization of CK14 and CK8/18 was captured with ImageJ. Scale bars: 100 μm. (See the quantification in Supplementary Fig. 6A). (B) Representative images of drug-treated mammary glands stained for stem cell markers: alkaline phosphatase (AP) (top 2 rows), CD44 (middle 2 rows) or CD24 (bottom 2 rows). Nuclei were counterstained with DAPI (blue). Scale bars: 50 μm. (See the quantification in Supplementary Fig. 6B–D). (C) CK14 and CK8/18 staining of non-malignant MCF10A cells cultivated in 3D ECM in the presence of control (PBS), L-arginine or L-NAME for 3 weeks. Scale bars: 50 μm. (See the quantification in Supplementary Fig. 6E). (D) Drug-treated MCF10A cells in 3D cultures stained for AP (top 2 rows), CD44 (middle 2 rows) or CD24 (bottom 2 rows). Nuclei were counterstained with DAPI (blue). Scale bars: 50 μm. (See the quantification in Supplementary Fig. 6F–H). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31040372), licensed under a CC-BY license. Not internally tested by Novus Biologicals.