Key Product Details

Species Reactivity

Human

Applications

Sandwich ELISA, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4A3

Format

BSA Free
View all alpha-Fetoprotein/AFP Primary Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human alpha-fetoprotein.

Localization

Secreted

Specificity

No cross-reaction with human albumin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for alpha-Fetoprotein/AFP Antibody (4A3) - BSA Free

Application
Recommended Usage

Sandwich ELISA

1:100-1:2000
Application Notes
We recommend the following pair in sandwich immunoassay (capture-detection): NB110-7961 - NB120-10072. See Simple Western Antibody Database for Simple Western validation: separated by size; matrix was 12-230 kDa; detected by Chemiluminescence.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: alpha-Fetoprotein/AFP

Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.

Alternate Names

AFP, DSCAM2

Entrez Gene IDs

174 (Human)

Gene Symbol

AFP

UniProt

P02771 (Human)

Additional alpha-Fetoprotein/AFP Products

Product Documents for alpha-Fetoprotein/AFP Antibody (4A3) - BSA Free

Certificate of Analysis

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Product Specific Notices for alpha-Fetoprotein/AFP Antibody (4A3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for alpha-Fetoprotein/AFP Antibody (4A3) - BSA Free

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