alpha Glucosidase 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32554

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all alpha Glucosidase 2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human alpha Glucosidase 2. The exact sequence is proprietary.

Reactivity Notes

Bovine (89%), Pig (80%).

Localization

Endoplasmic reticulum, Golgi apparatus, Melanosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

107 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha Glucosidase 2 Antibody - BSA Free

alpha Glucosidase 2 Antibody - BSA Free

Western Blot: alpha Glucosidase 2 Antibody [NBP1-32554] -

Wild-type (WT) and alpha Glucosidase 2 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Glucosidase 2 antibody [N1N2], N-term (NBP1-32554) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: alpha Glucosidase 2 Antibody [NBP1-32554]

Immunocytochemistry/ Immunofluorescence: alpha Glucosidase 2 Antibody [NBP1-32554]

Immunocytochemistry/Immunofluorescence: alpha Glucosidase 2 Antibody [NBP1-32554] - Analysis of methanol-fixed HeLa, using alpha Glucosidase II antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-32554]

Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-32554]

Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-32554] - Human breast carcinoma. alpha Glucosidase II antibody [N1N2], N-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: alpha Glucosidase 2 Antibody [NBP1-32554]

Western Blot: alpha Glucosidase 2 Antibody [NBP1-32554]

Western Blot: alpha Glucosidase 2 Antibody [NBP1-32554] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: alpha Glucosidase 2 Antibody [NBP1-32554]

Western Blot: alpha Glucosidase 2 Antibody [NBP1-32554]

Western Blot: alpha Glucosidase 2 Antibody [NBP1-32554] - Sample(30 ug whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 7.5% SDS PAGE diluted at 1:1000.
Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-32554]

Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-32554]

Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-32554] - SW480 xenograft, using alpha Glucosidase II antibody at 1:500 dilution.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Applications for alpha Glucosidase 2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alpha Glucosidase 2

Glucosidase II is an endoplasmic reticulum localized enzyme that has an important function in the folding and maturation of glycoproteins. Glucosidase II is composed of two soluble proteins: a catalytic alpha-subunit and a beta-subunit of unknown function, both of which are highly conserved in mammals.

Alternate Names

Alpha-glucosidase 2, EC 3.2.1, EC 3.2.1.84, G2ANGLUII, Glucosidase II subunit alpha, glucosidase, alpha; neutral AB, GluII, KIAA0088alpha glucosidase II alpha subunit, neutral alpha-glucosidase AB

Entrez Gene IDs

23193 (Human)

Gene Symbol

GANAB

UniProt

Q14697 (Human)

Additional alpha Glucosidase 2 Products

Product Documents for alpha Glucosidase 2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for alpha Glucosidase 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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